NANOPORE Technologies Sequencing Auxiliary Vials V14 for 12 Rxns

The ONT Sequencing Auxiliary Vials V14 kit is designed to enhance flexibility in sequencing workflows, particularly for users who wish to split, extend, or reuse libraries across multiple flow cells. This kit complements various V14 sequencing kits and protocols and ensures optimal use of resources when working with subsets of barcodes or when topping up flow cells.

Key Features

1. Enhanced Workflow Flexibility:
   • Enables splitting a prepared library across multiple flow cells.
   • Supports adding a new library onto a previously washed flow cell.
   • Allows topping up of flow cells with additional library material.
   • Maximizes efficiency by making appropriate use of unused barcodes, especially for smaller runs.
2. Compatibility:
   • Exclusively compatible with V14 chemistry kits, ensuring streamlined integration with ONT’s latest sequencing workflows.
   • Not suitable for the Ultra-Long DNA Sequencing Kit V14, which requires specific reagents.
3. Pack Size:
   • Sufficient reagents for 12 reactions, making it ideal for smaller or incremental sequencing experiments.

What’s in the Box

Compatibility

Kits:

The Auxiliary Vials V14 kit is compatible with the following V14 chemistry kits:

• Ligation Sequencing Kits: SQK-LSK114, SQK-LSK114-XL.
• Rapid Kits: SQK-RBK114.24, SQK-RBK114.96, SQK-RAD114.
• Native Barcoding Kits: SQK-NBD114.24, SQK-NBD114.96.
• PCR Barcoding Kits: SQK-RPB114.24, SQK-PCB114.24.
• Specialized Kits: 16S Barcoding Kit (SQK-16S114.24), cDNA-PCR Sequencing Kits (SQK-PCS114), Multiplex Ligation Sequencing Kit XL V14 (SQK-MLK114.96-XL).
• Auxiliary Kits: Flow Cell Wash Kit (EXP-WSH004), Flow Cell Priming Kit (EXP-FLP004/FLP004-XL), Rapid Adapter Auxiliary V14 (EXP-RAA114), Native Barcoding Auxiliary Kit V14 (EXP-NBA114).

Flow Cells:

• R10.4.1 flow cells:
  • FLO-MIN114
  • FLO-PRO114M

Devices:

• MinION Mk1B
• MinION Mk1C
• GridION
• PromethION

Shipping and Storage

• Shipping Conditions:
  • Shipped at 2–8°C in temperature-controlled, environmentally friendly packaging.
• Storage Conditions:
  • Long-term storage at -20°C.
  • Product deemed usable for 3 months from receipt.

Applications

1. Split Libraries:
   • Ideal for users wishing to run the same library across multiple flow cells to maximize resource usage.
2. Flow Cell Reuse:
   • Supports workflows where washed flow cells are reused with new or additional libraries.
3. Incremental Topping Up:
   • Enables topping up flow cells during sequencing for enhanced throughput.
4. Small-Scale Experiments:
   • Tailored for users running small subsets of barcodes, making efficient use of resources.

Advantages

• Cost-Effective:
  • Allows efficient use of reagents and flow cell capacity.
• Flexible Usage:
  • Compatible with multiple ONT kits and devices.
• Improved Workflow Efficiency:
  • Supports incremental and split-library workflows, ensuring minimal waste.

Safety and Legal Notes

• Safety Data Sheets provided for proper handling.
• Not intended for clinical diagnostic use.

The Sequencing Auxiliary Vials V14 kit is an essential addition for researchers using ONT’s V14 sequencing kits. It enables flexible, efficient, and cost-effective workflows, ensuring compatibility with various ONT platforms and experiments.