Thermo Scientific™ RNase A, DNase and protease-free (10 mg/mL)

Thermo Scientific™ RNase A, DNase and protease-free is a highly purified endoribonuclease enzyme that specifically degrades single-stranded RNA at C and U residues. This enzyme plays a critical role in RNA digestion, particularly for removing RNA from DNA preparations or protein samples during nucleic acid isolation procedures.

RNase A cleaves the phosphodiester bond between the 5'-ribose of a nucleotide and the phosphate group attached to the 3'-ribose of an adjacent pyrimidine nucleotide. The resulting 2', 3'-cyclic phosphate is hydrolyzed to form the corresponding 3'-nucleoside phosphate. This process makes RNase A essential for applications that require RNA degradation while ensuring the integrity of DNA during molecular biology procedures.

Key Features:

1. RNase A, DNase and Protease-Free:
   • This enzyme is free from DNase and protease activity, ensuring that RNA is selectively cleaved without affecting DNA or proteins.
2. Specific for Single-Stranded RNA:
   • RNase A cleaves single-stranded RNA at C and U residues, making it ideal for RNA removal from recombinant protein preparations or DNA.
3. Applications:
   • Plasmid and genomic DNA preparation
   • RNA removal from recombinant protein preparations
   • Ribonuclease protection assays, used with RNase T1
   • Mapping single-base mutations in DNA or RNA
4. Stable and Easy to Use:
   • RNase A is active at a wide range of reaction conditions, making it versatile for various applications. It does not require pre-activation or heating.
5. Wide Range of Concentration:
   • The recommended concentration of RNase A is 1 to 100 μg/mL, depending on the application.
6. Optimal for DNA/RNA Applications:
   • At low salt concentrations (0 to 100 mM NaCl), RNase A cleaves single-stranded RNA, double-stranded RNA, and the RNA strand in RNA-DNA hybrids. At 0.3 M NaCl or higher, it specifically cleaves single-stranded RNA.
7. Storage and Stability:
   • Store at -20°C for long-term stability.

Specifications:

Applications:

1. Plasmid and Genomic DNA Preparation:
   • RNase A is widely used for removing RNA from DNA preparations, ensuring high-quality, RNA-free DNA.
2. Recombinant Protein Preparation:
   • RNase A helps eliminate RNA contamination during the preparation of recombinant proteins, improving protein yield and purity.
3. Ribonuclease Protection Assays:
   • RNase A is used in RNA protection assays, which help protect RNA sequences during analysis.
4. Mutation Mapping:
   • Used in studies to map single-base mutations in DNA and RNA by ensuring complete RNA degradation without affecting the DNA.
5. Molecular Biology Applications:
   • Ideal for general RNA digestion in molecular biology and genomics to facilitate downstream applications like PCR, cloning, and nucleic acid analysis.

Related Products:

• Thermo Scientific™ RNA Extraction Kits
• Thermo Scientific™ DNA Isolation Kits
• Thermo Scientific™ Protein Lysis Buffers
• Thermo Scientific™ RNase T1

Target Information:

RNase A is a nuclease enzyme that cleaves single-stranded RNA at C and U residues. It is derived from the bovine pancreas and has applications in various molecular biology techniques such as RNA isolation, DNA/RNA purification, and RNA removal.

The Thermo Scientific™ RNase A, DNase and Protease-Free is an essential tool for RNA removal, DNA/RNA extraction, and protein preparation. With its high purity, stability, and broad application range, it is ideal for use in molecular biology and genomics studies. It ensures clean, high-quality nucleic acids for downstream applications like PCR, sequencing, and cloning.