Invitrogen™ Human LBP ELISA Kit

The Invitrogen Human LBP ELISA Kit is a highly sensitive and specific sandwich ELISA designed for the quantification of Lipopolysaccharide-Binding Protein (LBP) in human serum, plasma, and supernatant samples. This kit offers reliable and reproducible results, making it ideal for immunological and infectious disease research.

Key Features:

1. High Sensitivity and Wide Assay Range:
   • Analytical sensitivity: 0.82 ng/mL.
   • Assay range: 0.82–200 ng/mL.
2. Sample Efficiency:
   • Requires only 50 µL of sample per well, minimizing sample consumption.
3. Precision:
   • Intraassay CV: <10%.
   • Interassay CV: <12%.
4. Convenient Format:
   • Pre-coated 96-well plate enables efficient setup and high reproducibility.
5. Specificity:
   • Exclusively detects both natural and recombinant human LBP, ensuring accurate quantification.
6. Time-Saving Protocol:
   • Hands-on time: 1 hr 20 min.
   • Total time-to-result: 4 hr 45 min.
7. Comprehensive Kit Components:
   • Includes pre-coated 96-well plate, standards, biotinylated detection antibody, HRP conjugate, buffers, chromogen, and adhesive covers.

Specifications:

Applications:

1. Infectious Disease Research:
   • Quantifies LBP levels to study responses to gram-negative bacterial infections and sepsis.
2. Immunology Studies:
   • Investigates the role of LBP in the acute-phase immune response and monocyte activation.
3. Biomarker Analysis:
   • Measures LBP as a potential biomarker for immune activation in infectious and inflammatory diseases.
4. Drug Development:
   • Assesses the impact of therapeutic interventions targeting LBP-mediated pathways.

Principle of the Assay:

1. Sandwich ELISA Method:
   • A pre-coated monoclonal antibody captures LBP from the sample.
   • A biotinylated detection antibody binds the captured LBP.
   • HRP-conjugated streptavidin reacts with the biotinylated antibody.
   • TMB substrate is added to produce a blue color, which turns yellow after adding stop solution.
2. Quantification:
   • The color intensity is directly proportional to the LBP concentration and is measured using a colorimetric microplate reader.

Kit Components:

• Pre-coated 96-well plate.
• Standards for generating a calibration curve.
• Biotinylated detection antibody.
• Streptavidin-HRP conjugate.
• Assay diluent concentrate.
• Wash buffer.
• Chromogen (TMB substrate).
• Stop solution.
• Adhesive plate covers.

Advantages:

1. High Sensitivity:
   • Detects low levels of LBP, making it suitable for early-stage research or low-abundance samples.
2. Efficient Workflow:
   • Hands-on and total assay times are minimized, enabling high-throughput studies.
3. Comprehensive Kit:
   • Includes all necessary reagents for easy setup and execution.
4. Reliable and Reproducible:
   • Certified precision ensures consistent results across replicates and experiments.

Storage and Handling:

• Store all reagents at 2–8°C to maintain stability.
• Ensure all components are equilibrated to room temperature before use.
• Avoid repeated freeze-thaw cycles.

Background Information:

• Protein Name: Lipopolysaccharide-Binding Protein (LBP).
• Function: LBP plays a critical role in the acute-phase immune response to gram-negative bacterial infections by binding LPS and interacting with CD14 receptors.
• Gene Aliases: BPIFD2, LBP.
• Chromosomal Location: Chromosome 20.
• UniProt ID: P18428.

The Invitrogen Human LBP ELISA Kit is an invaluable tool for studying the immune response to bacterial infections and the role of LBP in inflammation. Its sensitivity, specificity, and reliable performance make it a versatile choice for researchers in immunology, microbiology, and infectious disease.