Invitrogen™ Human Hepatitis B Virus Surface Antigen ELISA Kit, 96 Tests

High-Sensitivity ELISA Kit for Quantitative Detection of HBsAg in Human Samples

The Invitrogen™ Human Hepatitis B Virus Surface Antigen (HBsAg) ELISA Kit is a highly specific and sensitive sandwich ELISA assay designed for the quantitative measurement of HBsAg in human serum, plasma, cell lysates, and tissue homogenates.

This 96-well microplate-based immunoassay is ideal for virology research, vaccine development, and hepatitis B studies. With an analytical sensitivity of 0.19 ng/mL, this kit enables accurate and reproducible detection of HBsAg levels in various biological fluids.

Key Features & Benefits

✅ Quantitative Detection of HBsAg – Measures Hepatitis B Virus Surface Antigen in human samples

✅ High Sensitivity (0.19 ng/mL) – Detects low levels of HBsAg with high accuracy

✅ Broad Assay Range (0.31 – 20 ng/mL) – Suitable for various experimental applications

✅ Validated for Multiple Sample Types – Compatible with serum, plasma, cell lysates, and tissue homogenates

✅ 96-Well Pre-Coated Microplate Format – Allows high-throughput screening

✅ Biotin-Streptavidin HRP System – Provides high specificity & signal amplification

✅ Fast & Easy Protocol (2 hr 30 min Total Time) – Reduces hands-on time for efficient research workflows

✅ Validated for Sensitivity, Specificity & Reproducibility – Ensures high precision and low inter-assay variability (<10%)

✅ For Research Use Only (RUO) – Designed for scientific and laboratory research purposes

Technical Specifications

Kit Components

✔ Pre-Coated 96-Well Plate (HBsAg-Specific Capture Antibody)

✔ Standard (Recombinant HBsAg Antigen, Lyophilized)

✔ Biotinylated Detection Antibody

✔ HRP Conjugate

✔ Standard & Sample Diluent

✔ Biotinylated Detection Antibody Diluent

✔ HRP Conjugate Diluent

✔ Wash Buffer

✔ TMB Substrate

✔ Stop Solution

✔ Plate Sealer

Principle of the Assay

The Human Hepatitis B Virus Surface Antigen (HBsAg) ELISA Kit is based on a solid-phase sandwich enzyme-linked immunosorbent assay (ELISA).

1️⃣ Capture Antibody Coating – The microplate wells are pre-coated with a target-specific antibody that binds to HBsAg in the sample.

2️⃣ Sample Addition & Incubation – The test sample is added to the wells, and HBsAg binds to the immobilized antibody.

3️⃣ Detection Antibody Binding – A biotinylated secondary antibody binds to a different epitope of HBsAg, forming a sandwich complex.

4️⃣ HRP Conjugate & Signal Generation – HRP-conjugated streptavidin is added, followed by TMB substrate, producing a colorimetric signal.

5️⃣ Stop Reaction & Read Absorbance – The reaction is stopped using Stop Solution, and absorbance is measured at 450 nm using a microplate reader.

The intensity of the color is proportional to the concentration of HBsAg in the sample.

Compatible Instruments

✔ Colorimetric Microplate Readers

✔ Automated ELISA Workstations

✔ Plate Readers with 450 nm Detection

Applications

🔬 Virology Research & Hepatitis B Studies – Detects HBsAg levels in clinical and research samples

🦠 Vaccine Development & Immunogenicity Testing – Evaluates immune responses in vaccine trials

🧪 Infectious Disease Research – Used in HBV pathogenesis & antiviral drug studies

⚕ Public Health & Epidemiological Surveillance – Assesses HBV prevalence in high-risk populations

🛠 Pharmaceutical & Drug Discovery – Supports HBV-targeted therapeutic development

Usage Guidelines

1️⃣ Prepare Reagents & Samples – Bring all components to room temperature before use.

2️⃣ Load Samples & Standards – Add 100 µL of sample or standard into each well.

3️⃣ Incubate & Wash – Allow binding and perform multiple wash steps to remove unbound material.

4️⃣ Add Biotinylated Detection Antibody & HRP Conjugate – Incubate and wash again.

5️⃣ Develop Color Reaction – Add TMB substrate and allow color to develop.

6️⃣ Stop & Read Absorbance – Stop reaction and measure optical density at 450 nm.

Regulatory & Safety Information

⚠️ For Research Use Only (RUO). Not intended for diagnostic or clinical applications.

⚠️ Handle Biological Samples with Caution. Follow biosafety guidelines (BSL-2 or higher) for infectious agents.

⚠️ Dispose of Used Materials Safely. Follow laboratory waste disposal regulations for hazardous substances.

Why Choose the Invitrogen™ HBsAg ELISA Kit?

🔹 Highly Sensitive & Specific Detection (0.19 ng/mL) – Ensures accurate & reproducible results

🔹 Validated for Serum, Plasma & Cell Lysates – Offers broad sample compatibility

🔹 Rapid & Efficient Workflow (2 hr 30 min Total Time) – Reduces laboratory turnaround time

🔹 Robust Assay Performance (Inter-Assay CV <10%) – Delivers consistent & high-quality data

🔹 Ideal for HBV Research, Epidemiology & Vaccine Development – Supports critical virology studies

For bulk orders, technical support, or additional information, contact LabMart Limited today.