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Thermo Scientific™ Oxoid™ PBP2' Latex Agglutination Test Kit
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for detection of PBP2' as an aid in identifying methicillin resistance in both Staphylococcus aureus (MRSA) and coagulase-negative Staphylococci.
Specifications:
| Application | Serology |
| Storage Temperature | 2-8°C |
| Product Type | Microbiology Reagent |
| Product Brand | Thermo Fisher Scientific™ |
| Product Grade | Microbiology grade |
The Thermo Scientific™ Oxoid™ PBP2' Latex Agglutination Test Kit is a rapid and reliable diagnostic tool designed for the detection of the low-affinity penicillin-binding protein PBP2' (encoded by the mecA gene), which is a hallmark of methicillin-resistant Staphylococcus aureus (MRSA) and coagulase-negative staphylococci.
This qualitative test aids in the differentiation of methicillin-resistant strains from other staphylococcal species, supporting clinicians in choosing appropriate treatment options for patients suspected of MRSA infection or colonization.
Key Features:
- Rapid Testing:
- Provides results in minutes, streamlining diagnostic workflows.
- Reduces time to detect MRSA compared to conventional methods.
- Comprehensive Coverage:
- Detects PBP2' in MRSA and coagulase-negative staphylococci.
- Designed for isolates grown on agar, ensuring accurate results.
- User-Friendly:
- Latex slide agglutination format is straightforward and requires no specialized equipment.
- Ideal for professional healthcare laboratory settings.
- Applications:
- Diagnostic Workflow: Helps clinicians make informed decisions on antibiotic therapy.
- Infection Control: Facilitates rapid identification of resistant strains to curb spread in healthcare environments.
- Intended Use:
- Non-automated and designed for professional use only.
- Not a companion diagnostic.
Specifications:
| Feature | Details |
|---|---|
| Product Type | Latex Agglutination Test Kit |
| Target | Low-affinity penicillin-binding protein PBP2' (mecA gene) |
| Organisms | Staphylococcus aureus (MRSA), Coagulase-negative Staphylococci |
| Applications | Healthcare, Infection Control |
| Unit Size | Each |
| Quantity | 50 Tests/Kit |
| Test Time | Rapid, qualitative results |
| Intended Use | Differentiation of methicillin resistance |
| Storage Conditions | Store at 2–8°C |
Test Procedure:
- Preparation:
- Ensure all reagents are at room temperature before use.
- Grow the bacterial isolate on a suitable agar medium.
- Reagent Application:
- Place a drop of the latex reagent on the test slide.
- Mix the isolate suspension with the latex reagent.
- Observation:
- Gently rotate the slide and observe for agglutination.
- Positive result: Presence of visible agglutination indicates PBP2'.
- Negative result: No visible agglutination.
- Result Interpretation:
- Positive test confirms methicillin resistance due to PBP2'.
- Negative test rules out resistance associated with mecA-mediated PBP2'.
Quality Control:
| Control Strains | Expected Results |
|---|---|
| Staphylococcus aureus ATCC® 43300 | Positive |
| Staphylococcus epidermidis ATCC® 12228 | Positive |
| Staphylococcus aureus ATCC® 25923 | Negative |
Comparison with Other Methods:
| Feature | Thermo Scientific™ Oxoid™ PBP2' | A | B |
|---|---|---|---|
| Test Type | Latex Agglutination | PCR-based test | ELISA-based test |
| Time to Result | Minutes | Several hours | 2–3 hours |
| Ease of Use | High | Moderate | Moderate |
| Cost-Effectiveness | High | Expensive | Moderate |
| Equipment Requirement | None | Thermal cycler | ELISA reader |
Storage Instructions:
- Store at 2–8°C in a refrigerator.
- Do not freeze the test kit.
- Keep the kit away from direct sunlight and ensure it is sealed when not in use.
The Thermo Scientific™ Oxoid™ PBP2' Latex Agglutination Test Kit is a rapid, efficient, and reliable tool for the identification of methicillin resistance in staphylococcal isolates. With its ease of use and compatibility with standard laboratory workflows, it is a valuable addition to any diagnostic laboratory focusing on infection control and antibiotic stewardship.