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Thermo Scientific™ Oxoid™ Legionella Agar Base
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Specifications:
| Application | Microbiology | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Culture Medium | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™ | Target Organism Class | Legionella spp. |
| Product Grade | Microbiology grade | Formula | For 20.0L medium |
The Thermo Scientific™ Oxoid™ Legionella CYE Agar Base (Dehydrated) is a specialized medium designed for the isolation and recovery of Legionella spp. from environmental samples and clinical specimens. When used with the Legionella Growth Supplement (SR0251A), this medium provides optimal conditions for the recovery of Legionellaceae in short incubation periods, ensuring accurate and reliable detection.
Key Features
- Non-Selective Medium:
- Promotes the growth of Legionella spp., providing the necessary nutrients for their recovery.
- Legionella Growth Supplement:
- Used with SR0251A, which supplies essential growth factors for Legionella species.
- High Recovery Efficiency:
- Ensures optimal recovery of Legionella from both environmental samples and clinical specimens.
- ISO-Compliant:
- Meets international standards for Legionella detection in microbiological workflows.
- Broad Application:
- Suitable for testing water systems, cooling towers, and clinical samples for Legionella spp..
Applications
1. Clinical Microbiology
- Isolation of Legionella pneumophila, the primary causative agent of Legionnaires’ disease and Pontiac fever, from respiratory samples.
2. Environmental Microbiology
- Detection of Legionella spp. in water systems, cooling towers, and industrial environments to ensure safety compliance.
3. Public Health and Research
- Studies on Legionella ecology and its role in outbreaks of legionellosis.
Specifications
| Attribute | Details |
|---|---|
| Product Type | Agar |
| Form | Powder |
| Description | Legionella CYE Agar Base |
| Applications | Clinical, environmental, and research microbiology |
| Quantity | 500g |
| Yield | 20.0L of prepared medium |
| Compliance | ISO Standards |
Preparation Instructions
- Suspend: Suspend 25g of Legionella CYE Agar Base powder in 500mL of distilled water.
- Mix and Heat: Heat with frequent agitation until fully dissolved.
- Sterilize: Autoclave at 121°C for 15 minutes.
- Cool and Supplement: After cooling to 45–50°C, aseptically add the Legionella Growth Supplement (SR0251A).
- Dispense: Pour the medium into sterile Petri dishes and allow to solidify.
Advantages
- High Sensitivity: Supports the growth of Legionella spp., including L. pneumophila, with reliable recovery rates.
- Versatile Applications: Suitable for both clinical and environmental testing workflows.
- ISO-Compliant: Meets international microbiology standards for Legionella detection.
- Easy to Prepare: Simplifies laboratory workflows with consistent and reproducible results.
- Cost-Effective: High yield of 20L per 500g, making it ideal for routine and large-scale testing.
Diagnostic Features
| Organism | Growth Characteristics |
|---|---|
| Legionella pneumophila | Robust growth with colony morphology for identification. |
| Other Legionella spp. | Consistent recovery with supplement support. |
Storage and Stability
- Storage: Store the dehydrated medium at 10–30°C in a dry environment.
- Shelf Life: Refer to product label for expiration date.
Packaging
- Product: Legionella CYE Agar Base (Dehydrated)
- Catalog Number: CM1203B
- Quantity: 500g
- Yield: 20.0L of prepared medium
The Thermo Scientific™ Oxoid™ Legionella CYE Agar Base (Dehydrated) is a reliable and effective medium for the detection and isolation of Legionella spp. Its use with the Legionella Growth Supplement (SR0251A) ensures accurate and efficient recovery of Legionella from clinical and environmental samples, making it an indispensable tool for microbiology laboratories focused on public health and environmental monitoring.