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Diagenode MicroChIP DiaPure columns, 50 rxns
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Specifications:
| Application | Next Generation Sequencing, NGS Library Preparation |
| Storage Temperature | 2-8°C |
| Product Type | NGS library Prep kits |
| Product Brand | Hologic Diagenode |
| Product Grade | Molecular Biology |
Diagenode’s MicroChIP DiaPure columns have been optimized for the purification and elution of very low amounts of DNA. This rapid method has been validated for epigenetic applications like low input ChIP (e.g. using the True MicroChIP kit) and CUT&Tag (e.g. using Diagenode’s pA-Tn5), but is also compatible with many other applications. The DNA can be eluted at high concentrations in volumes down to 6 μl and it is suitable for any downstream application (e.g. NGS).
Benefits of the MicroChIP DiaPure columns:
- Optimized for the purification of very low DNA amounts
- Fast and easy protocol
- Non-toxic
- Validated for ChIP and Cut&Tag
Examples of results
MicroChIP DiaPure columns after ChIP
Successful ChIP-seq results generated on 50,000 of K562 cells using True MicroChIP technology. ChIP has been performed accordingly to True MicroChIP protocol (Diagenode, Cat. No. C01010130), including DNA purification using the MicroChIP DiaPure columns. For the library preparation the MicroPlex Library Preparation Kit (Diagenode, Cat. No. C05010001) has been used. The below figure shows the peaks from ChIP-seq experiments using the following Diagenode antibodies: H3K4me1 (C15410194), H3K9/14ac (C15410200), H3K27ac (C15410196) and H3K36me3 (C15410192).
Figure 1: Integrative genomics viewer (IGV) visualization of ChIP-seq experiments using 50,000 of K562 cells.
MicroChIP DiaPure columns after CUT&Tag
Successful CUT&Tag results showing a low background with high region-specific enrichment has been generated using 50.000 of K562 cells, 1 µg of H3K27me3 antibody (Diagenode, Cat. No. C15410069) and proteinA-Tn5 (1:250) (Diagenode, Cat. No. C01070001). 1 µg of IgG (Diagenode, Cat. No. C15410206) was used as negative control. Samples were purified using the MicroChIP DiaPure columns or phenol-chloroform purification. The below figure presenst the comparison of two purification methods.
Figure 2: Integrative genomics viewer (IGV) visualization of CUT&Tag experiments: MicroChIP DiaPure columns vs phenol-chloroform purification using the H3K27me3 antibody.