Applied Biosystems™ TaqPath™ 1-Step Multiplex Master Mix (No ROX)

The TaqPath™ 1-Step Multiplex Master Mix (No ROX) is a versatile, single-tube, 4X RT-qPCR mix optimized for sensitive and reproducible detection of up to four RNA or DNA targets in a single reaction. Designed for diagnostics, biopharmaceutical applications, and high-throughput workflows, this master mix offers exceptional performance even in challenging samples with PCR inhibitors. Manufactured under ISO13485 standards, it ensures lot-to-lot consistency for reliable results.

Key Features:

1. High Sensitivity:
   • Detects low-copy targets with consistent and reproducible cycle threshold (CT) values.
   • Higher-concentration 4X mix allows for increased sample input, enhancing sensitivity in low-volume reactions.
2. Wide Dynamic Range:
   • Supports a six-log dynamic range for RNA and DNA targets, providing flexibility for both nucleic acid types.
   • Enables testing for RNA and DNA viruses with a unified protocol to streamline workflows.
3. Optimized for Multiplexing:
   • Detect up to four targets simultaneously without requiring a passive reference dye (No ROX).
   • Compatible with JUN or similar emission wavelength dyes, expanding multiplexing options.
4. Inhibitor Tolerance:
   • Robust proprietary formulation ensures reliable performance in the presence of PCR inhibitors such as heparin, hematin, and EDTA.
   • Ideal for complex clinical and biopharmaceutical samples.
5. Advanced Manufacturing Standards:
   • Produced under ISO13485-certified quality management and cGMP principles.
   • Stringent production controls ensure consistency across lots, minimizing variation.
6. Compliance and Support:
   • Comprehensive compliance package includes Certificates of Analysis (COA), product stability statements, and cGMP adherence details.
   • General Purpose Reagent (GPR) labeled for laboratory use.

Specifications:

Packaging Options:

Applications:

• Viral pathogen detection.
• Gene expression analysis.
• High-throughput RT-qPCR workflows.
• Diagnostics and biopharmaceutical quality control.

Contents:

• Thermostable Fast DNA Polymerase
• Thermostable MMLV Reverse Transcriptase
• dNTPs
• UNG (Uracil-N-glycosylase)

Advantages:

1. Multiplex Efficiency:
   • Detect multiple targets or controls simultaneously for enhanced throughput and reduced assay complexity.
2. Robust in Complex Samples:
   • Handles inhibitors effectively, ensuring reliable results across challenging sample types.
3. Reproducible Performance:
   • Manufactured under stringent controls to deliver consistent CT values across multiple assays.

Storage and Stability:

• Store at -10°C to -30°C to maintain integrity and performance.

The TaqPath™ 1-Step Multiplex Master Mix (No ROX) offers a reliable, flexible, and efficient solution for real-time qPCR workflows. Its optimized performance for RNA and DNA targets, combined with high inhibitor tolerance and multiplexing capability, makes it an excellent choice for demanding applications in molecular diagnostics and research.