Applied Biosystems™ TaqMan™ SNP Genotyping Assay, human

Applied Biosystems TaqMan SNP Genotyping Assays use TaqMan 5´‑nuclease chemistry to amplify and detect specific polymorphisms in purified genomic DNA samples. Each assay enables genotyping of individuals for a single nucleotide polymorphism (SNP) and consists of two sequence-specific primers and two TaqMan minor groove binder (MGB) probes with non-fluorescent quenchers (NFQ). One probe is labeled with VIC dye to detect the Allele 1 sequence; the second probe is labeled with FAM dye to detect the Allele 2 sequence.

Our predesigned TaqMan SNP Genotyping Human Assays are a genome-wide collection of millions of human assays, including common 1,000 Genome SNPs, HapMap SNPs, and coding SNPs.

Benefits:

• Proven—gold-standard TaqMan chemistry and robust assay designs deliver accurate, reproducible, and reliable results

• Easy—convenient single-tube format and simple workflow provide an easy path to trusted results; no optimization required

• Relevant—extensive collection of predesigned human assays offers direct access to content that is relevant to your research

• Tested—all human SNP genotyping assays are functionally tested to ensure allelic discrimination

TaqMan SNP Genotyping Assays require only three reaction components for PCR: purified genomic DNA (1–20 ng), the assay solution, and TaqMan Genotyping Master Mix (or another compatible master mix) (sold separately).