Applied Biosystems™ TaqMan™ MGB Probe

Applied Biosystems™ TaqMan™ MGB (Minor Groove Binder) probes are advanced dual-labeled probes designed for real-time PCR (qPCR) applications, leveraging TaqMan chemistry. These probes provide exceptional precision and sensitivity, making them suitable for a wide range of molecular biology applications.

Key Features

1. Dual-Labeled Design:
   • 5' End: Fluorescent reporter dye (options: FAM, VIC, TET, NED).
   • 3' End: Nonfluorescent quencher (NFQ) to minimize background fluorescence.
2. MGB Moiety:
   • Enhances the melting temperature (Tm) of the probe.
   • Stabilizes probe-target hybridization.
   • Enables the use of shorter probes for increased specificity and better sequence discrimination.
3. NFQ Quencher:
   • Reduces background fluorescence.
   • Improves data precision and sensitivity compared to non-MGB NFQ probes.
4. Customizable Options:
   • Available in various scales (6,000 pmol, 20,000 pmol, 50,000 pmol).
   • Tailored dyes, concentrations, and large-scale options via the TaqMan Custom Assay and Oligo Service.
5. High Purity:
   • HPLC-purified probes ensure reproducibility and accuracy.
6. Compatibility:
   • Designed for a wide range of Applied Biosystems real-time PCR instruments, including:
     • QuantStudio™ Systems
     • StepOne™/StepOnePlus™
     • 7500/7500 Fast Systems
     • 7900HT System
     • ViiA™ 7 System

Benefits

• Improved Specificity: Shorter probe length combined with MGB moiety enables precise targeting and discrimination of sequences.
• Enhanced Sensitivity: NFQ ensures lower background fluorescence, enabling more accurate quantification, especially in multiplex assays.
• Versatility: Suitable for singleplex or multiplex qPCR applications.
• Customizability: Available in liquid format and customizable to meet specific experimental needs.

Applications

• Real-Time PCR (qPCR):
  • Gene expression studies.
  • SNP genotyping.
  • Mutation detection.
  • Pathogen detection.
  • Molecular diagnostics (research use only).

Specifications

Use Cases

1. Multiplex PCR:
   • Detect up to four targets simultaneously with dyes like FAM, VIC, TET, and NED.
   • Ideal for complex pathogen detection and gene expression profiling.
2. Custom Applications:
   • Tailored probe design for unique sequences.
   • Pre-formulated TaqMan assays available.
3. High-Throughput Experiments:
   • Efficient in large-scale genomic studies requiring precise quantification.

Ordering Information

• Catalog Numbers:
  • 4316034: 6,000 pmol
  • 4316033: 20,000 pmol
  • 4316032: 50,000 pmol

For Research Use Only. Not for use in diagnostic procedures. Ensure proper handling and storage for optimal probe performance.