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Applied Biosystems™ MagMAX™ Pure Bind Beads
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Specifications:
| Application | Next Generation Sequencing | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Sequencing Reagents | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The MagMAX Pure Bind Beads are advanced magnetic beads designed for efficient PCR cleanup and DNA purification, providing superior recovery of DNA fragments larger than 90 bp. These beads streamline workflows by removing impurities such as dNTPs, primers, primer-dimers, and salts, making them an excellent choice for Next-Generation Sequencing (NGS) and PCR applications.
Key Features and Benefits
- High DNA Recovery:
- Achieves >90% recovery for DNA fragments larger than 90 bp.
- Consistent and reliable results for both GC-rich and standard PCR products.
- Workflow Compatibility:
- Integrates seamlessly with manual or automated workflows.
- Optimized for use with KingFisher automated purification systems (Apex and Flex).
- Simplified Setup:
- One-bottle solution eliminates the need for multiple reagents.
- Short protocol (~20 minutes) reduces hands-on time.
- Scalability:
- Compatible with various sample volumes, allowing flexibility for both small-scale and high-throughput applications.
- Sustainability:
- Shipped and stored at room temperature, reducing energy usage and cold storage requirements.
- Streamlines operations with a grab-and-go design.
- High-throughput Ready:
- Process up to 60 µL of PCR sample volumes using KingFisher 96 deep-well plates.
- BindIx and BindIt software available for protocol optimization.
Specifications
| Attribute | Details |
|---|---|
| Form | Beads in suspension |
| GC-Rich PCR Performance | High |
| Fragment Recovery | >90% for fragments >90 bp |
| Applications | NGS cleanup, PCR cleanup, PCR purification, DNA purification, sequencing |
| Processing Mode | Manual or automated (e.g., KingFisher systems) |
| Test Time | ~20 minutes |
| Volumes Available | 5 mL, 50 mL, 250 mL |
| Unit Size | 250 mL |
| Storage Conditions | Room temperature |
Applications
- NGS Library Cleanup:
- Effective in removing impurities to ensure high-quality library preparation for sequencing.
- PCR Cleanup:
- Eliminates unincorporated primers, dNTPs, and other contaminants, improving downstream accuracy.
- Sequencing Workflow Optimization:
- Enhances target enrichment when used with Dynabeads Streptavidin for Target Enrichment.
- DNA Purification:
- Recovers high-quality DNA suitable for sensitive downstream applications.
Workflow
- Bind: DNA fragments bind to the magnetic beads.
- Wash: Beads are washed to remove contaminants.
- Elute: Purified DNA is eluted from the beads.
- Transfer: DNA is transferred for downstream applications.
Automation Ready
- Optimized for use with KingFisher systems:
- KingFisher Apex (Cat. No. 5400910).
- KingFisher Flex (Cat. No. 5400610).
- Reduces hands-on time while ensuring reproducible results.
Comparison of Applied Biosystems™ MagMAX™ Pure Bind Beads and Beckman Coulter™ Agencourt AMPure XP
| Feature | MagMAX Pure Bind Beads | AMPure XP |
|---|---|---|
| DNA Recovery | >90% for fragments >90 bp | 70–90% for fragments >100 bp |
| Fragment Size Range | Focused on >90 bp fragments | Allows for size selection with adjustable bead-to-sample ratio |
| Workflow Integration | Manual and automated workflows (optimized for KingFisher systems) | Manual and automated workflows |
| Storage Conditions | Room temperature | Requires 4°C |
| Applications | PCR cleanup, NGS cleanup, DNA purification, sequencing | PCR cleanup, NGS cleanup, DNA size selection |
| Size Selection Capability | No specific size selection protocol | Supports predictable size selection with bead-to-sample ratio |
| Processing Time | ~20 minutes | ~25–30 minutes |
| Volume Options | 5 mL, 50 mL, 250 mL | 5 mL, 60 mL, 450 mL |
| Technology | SPRI paramagnetic bead-based technology | SPRI paramagnetic bead-based technology |
| Automation Compatibility | KingFisher Apex, KingFisher Flex | Compatible with most liquid handling systems |
| Sustainability | Room temperature storage reduces energy use | Requires cold storage, higher energy demand |
| Recommended Platforms | KingFisher instruments, manual workflows | Broad compatibility with various platforms |
| Target Applications | High-throughput workflows, simplified protocols | Complex workflows requiring size selection |
Both MagMAX Pure Bind Beads and AMPure XP offer reliable solutions for DNA purification and PCR cleanup. The choice between them depends on specific laboratory needs:
- MagMAX Pure Bind Beads are advantageous for laboratories seeking high DNA recovery with the convenience of room temperature storage and integration with KingFisher automated systems.
- AMPure XP is suitable for applications requiring precise DNA size selection and is compatible with various genomic workflows, albeit with the necessity for cold storage.
Evaluating the specific requirements of your applications will guide the optimal selection between these two products.
MagMAX Pure Bind Beads are a reliable and efficient choice for laboratories seeking consistent and high-quality DNA purification.
- Pack Size: 50 mL 5 mL 250 mL