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Applied Biosystems™ Human GAPD (GAPDH) Endogenous Control (FAM™/MGB probe, non-primer limited)
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Specifications:
| Application | qPCR | ||
| Storage Temperature | -20°C | ||
| Product Type | Primers | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Applied Biosystems™ | TaqMan™ Gene Expression Assays
The Applied Biosystems™ Human GAPDH Endogenous Control (FAM™ Dye ⁄ MGB Probe, Non-Primer Limited) is a validated, ready-to-use control for normalizing gene expression data in real-time quantitative PCR (qPCR) workflows. This assay is designed for singleplex reactions and is ideal for comparing gene expression levels across different samples or treatment conditions using the GAPDH gene as a stable internal reference.
Product Highlights
- Probe Chemistry: FAM™-labeled TaqMan® MGB probe with non-primer limited PCR primers
- Validated for singleplex qPCR workflows
- Amplicon Size: 122 bp
- Gene Target: Human GAPDH (NM_002046.3, exon 3)
- Compatible with: Applied Biosystems™ 7900HT, 7500, 7300, 7000, and 7700 qPCR instruments
- Recommended Use: Normalization of cDNA template loading in gene expression assays
- Multiplexing: Not recommended (non-primer limited)
Key Specifications
| Parameter | Details |
|---|---|
| Target Gene | GAPDH (Human) |
| Accession Number | NM_002046.3 |
| Amplicon Size | 122 bp |
| Probe Label | FAM™ Dye / MGB Probe |
| Concentration | 20X Primer-Probe Mix |
| PCR Method | 2-step RT-qPCR |
| Format | Tube (Liquid) |
| Detection Method | Primer–Probe (TaqMan™ chemistry) |
| Storage Conditions | Room Temperature (during shipping) |
| Sustainability | Sustainable packaging |
Available Pack Sizes
| Catalog Number | No. of Reactions | Packaging |
|---|---|---|
| 4333764T | 125 Reactions | Tube |
| 4333764F | 500 Reactions | Tube |
| 4352934E | 2500 Reactions | Tube |
Applications
- Relative quantification of gene expression
- Normalization control in TaqMan™ gene expression workflows
- RNA and cDNA quality control
- Gene expression studies in human samples
- Use with inventoried, custom, or non-inventoried TaqMan™ assays
Multiplexing Note
This FAM™-labeled control is not primer limited and is intended for singleplex use only. For multiplex qPCR experiments, consider using VIC™-labeled primer-limited endogenous controls.
- Reactions: 500 rxn 2500 rxn 125 rxn