roQ QuEChERS Kits make sample preparation easy. roQ QuEChERS kits eliminates common problems seen with other QuEChERS kits by incorporating features such as trays to hold centrifuge tubes, easy pour salt packets, and stand-alone centrifuge tubes making the removal unwanted interferences such as organic acids, lipids, pigments, sugars, and more.

A Simple 2-Step Method

Step 1: Extraction 

Pesticides and analytes of interest must first be extracted from the sample. This process relies on the combination of organic solvent and various salts to partition the analytes from samples into an organic layer (typically acetonitrile).

Blend fruits or vegetables to be analyzed.Weigh blended sample.Add salts and acetonitrile.Shake tube for 1 minute.Centrifuge tube for <br>5 minutes.

Step 2: Dispersive SPE (dSPE) 

An aliquot of the organic layer from the extraction step is subjected to further clean up by dispersive SPE. This step selectively removes unwanted interferences such as lipids and pigments.

Add supernatant from extraction procedure into a roQ dSPE tube.Shake dSPE tube for 30 seconds.Centrifuge dSPE tube for 5 minutes.Analyze supernatant by GC or HPLC.

Method Recommendations for roQ

Step 1: Extraction

Pesticides and analytes of interest must first be extracted from the sample. This process relies on the combination of organic solvent and various salts to partition the analytes from samples into an organic layer (typically acetonitrile).

AOAC	ORIGINAL	EN
AOAC 2007.01 Method 6.0 g MgSO4, 1.5 g NaOAc KS0-8911	Non-Buffered Method 4.0 g MgSO4, 1.0 g NaCl KS0-8910 6.0 g MgSO4, 1.5 g NaCl KS0-8912	EN 15662 Method 4.0 g MgSO4, 1.0 g NaCl, 1.0 g SCTD, 0.5 g SCDS KS0-8909

*All roQ Extraction kits contain fifty easy-pour salt packets and fifty 50 mL stand-alone centrifuge tubes.

Step 2: Clean Up/dSPE**

	AOAC 2007.01	EN 15662
	1 mL	8 mL	1 mL	6mL
General	150 mg MgSO4, 50 mg PSA KS0-9511	1200 mg MgSO4, 400 mg PSA KS0-9515	150 mg MgSO4, 25 mg PSA KS0-9503	900 mg MgSO4, 150 mg PSA KS0-9507
Fats and Waxes	150 mg MgSO4, 50 mg PSA  50 mg C18E KS0-9512	1200 mg MgSO4, 400 mg PSA KS0-9515	150 mg MgSO4, 25 mg PSA KS0-9503	900 mg MgSO4, 150 mg PSA KS0-9507
Pigmented	150 mg MgSO4 50 mg PSA 50 mg GCB KS0-9513	1200 mg MgSO4, 400 mg PSA 400 mg GCB KS0-9517	150 mg MgSO4 25 mg PSA 2.5 mg GCB KS0-9505	900 mg MgSO4 150 mg PSA 15 mg GCB KS0-9509
Highly Pigmented	–	–	150 mg MgSO4 25 mg PSA 7.5 mg GCB KS0-9506	900 mg MgSO4 150 mg PSA 45 mg GCB KS0-9510
Pigments and Fats	150 mg MgSO4 50 mg PSA 50 mg GCB 50 mg C18E KS0-9514	1200 mg MgSO4 400 mg PSA 400 mg GCB 400 mg C18E KS0-9518	–	–

**All roQ dSPE kits contain pre-weighed sorbents/salts inside 2 mL or 15 mL centrifuge tubes.