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Specifications:
| Application | ELISA-Based Assays |
| Storage Temperature | 2-8°C |
| Product Type | Elisa Kit |
| Product Brand | abcam |
| Product Grade | Molecular Biology |
The Abcam NEFL ELISA Kit is a high-performance, single-wash SimpleStep ELISA® designed for the quantitative detection of Neurofilament Light Polypeptide (NEFL) in tissue extract samples. The assay is designed for research purposes and offers fast and reliable results within 90 minutes. It is particularly valuable in studies related to neurodegeneration, neuronal injury, and neurological diseases.
Key Features
- Fast and Efficient:
- Single-wash, 90-minute assay protocol for rapid analysis.
- Quantitative Measurement:
- Detects NEFL protein in tissue extracts with high sensitivity.
- High Specificity:
- Captures natural and recombinant human NEFL using monoclonal antibodies.
- SimpleStep ELISA® Technology:
- Pre-coated 96-well plates simplify the workflow and reduce handling steps.
- Broad Range of Applications:
- Suitable for analyzing neuronal health, neurofilament expression, and neurodegenerative diseases.
Background on NEFL
NEFL, or Neurofilament Light Polypeptide, is one of the three core neurofilament proteins, along with NEFM and NEFH. These proteins are critical for maintaining the structural integrity and caliber of neuronal axons. NEFL cooperates with other intermediate filament proteins like PRPH and INA to form neuronal networks, making it a key biomarker in neurological research.
Applications
- Studies of neurodegenerative diseases (e.g., ALS, Parkinson's, Alzheimer's).
- Investigation of neuronal injury and recovery.
- Research on axonal transport and cytoskeletal integrity.
- Quantification of neurofilament proteins in disease models.
Kit Contents
| Component | Quantity | Description |
|---|---|---|
| 10X Human NEFL Capture Antibody | 1 x 600 µL | Monoclonal antibody for capturing NEFL. |
| 10X Human NEFL Detector Antibody | 2 x 1 Vial | Biotinylated monoclonal antibody for NEFL detection. |
| 10X Wash Buffer PT (ab206977) | 1 x 20 mL | Wash buffer for plate washing steps. |
| 50X Cell Extraction Enhancer Solution (ab193971) | 1 x 1 mL | Enhances extraction of NEFL from samples. |
| 5X Cell Extraction Buffer PTR (ab193970) | 1 x 10 mL | Extraction buffer for preparing tissue extracts. |
| Antibody Diluent 5BI | 1 x 6 mL | Diluent for antibody preparation. |
| Human NEFL Lyophilized Recombinant Protein | 2 x 1 Vial | Standard for calibration curve preparation. |
| Plate Seals | 1 x 1 Unit | Adhesive seals for preventing evaporation. |
| Sample Diluent NS (ab193972) | 1 x 12 mL | Diluent for sample preparation. |
| SimpleStep Pre-Coated 96-Well Microplate (ab206978) | 1 x 1 Unit | Pre-coated plate for binding NEFL-specific antibodies. |
| Stop Solution | 1 x 12 mL | Stops enzymatic reaction for colorimetric readout. |
| TMB Development Solution | 1 x 12 mL | Substrate for HRP enzyme to produce a colorimetric signal. |
Advantages
- Speed: Complete assay in just 90 minutes.
- Ease of Use: Single-wash format with pre-coated plates reduces handling time.
- Precision: High sensitivity and low variability (Intra-assay CV <10%, Inter-assay CV <12%).
- Versatility: Suitable for use with tissue extracts and other cell lysate samples.
Technical Specifications
- Target Protein: NEFL (Neurofilament Light Polypeptide).
- Sample Type: Tissue extracts and cell lysates.
- Sensitivity: High sensitivity for precise quantification.
- Assay Range: Quantitative measurement across a broad range.
- Detection Method: Colorimetric (requires a microplate reader).
Alternative Names for NEFL
- NF-L
- Neurofilament light polypeptide
- 68 kDa neurofilament protein
- Neurofilament triplet L protein
- NF68
- NFL
Storage and Stability
- Store all components at 2°C to 8°C.
- Protect from light to maintain reagent integrity.
- Lyophilized recombinant protein and prepared plates are stable until expiry under recommended storage conditions.
This kit offers a robust and reliable solution for NEFL measurement, essential for advancing research in neurobiology and related fields.