0
Thermo Scientific™ MgCl2 (magnesium chloride) (25 mM), 5mL
Catalog No :
CAS Number :
Brand :
In Stock
Store at -20°C
Specifications:
| Application | PCR | ||
| Storage Temperature | -20°C | ||
| Product Type | PCR Reagent | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Thermo Scientific™ 25 mM Magnesium Chloride (MgCl₂) Solution is a high-purity PCR reagent designed to support DNA polymerase activity in polymerase chain reactions (PCR). Magnesium ions (Mg²⁺) are essential cofactors that interact with the DNA template, dNTPs, and Taq DNA Polymerase, optimizing PCR performance even for challenging molecular biology applications.
Key Features & Benefits
1. Optimized for PCR Performance
- Essential for DNA polymerase function in PCR, qPCR, and RT-PCR.
- Ensures accurate and high-fidelity DNA amplification by stabilizing enzyme-substrate interactions.
- Reliable for standard PCR applications, enhancing primer binding and nucleotide incorporation.
2. High-Quality Molecular Biology Grade
- Strict quality control for reproducible results in molecular biology applications.
- Manufactured under high-purity conditions to minimize contamination.
- Reliable for use in complex PCR reactions with low-template or GC-rich DNA.
3. Ready-to-Use, Pre-Diluted Concentration (25 mM)
- Convenient liquid format, eliminating the need for manual dilution.
- Easily adjustable for custom PCR reaction conditions.
- Supplied in 5 mL aliquots, suitable for multiple reactions.
Specifications
| Property | Details |
|---|---|
| Chemical Name | Magnesium Chloride (MgCl₂) |
| Concentration | 25 mM |
| Form | Liquid solution |
| Volume | 5 mL |
| Polymerase Compatibility | Taq DNA Polymerase |
| Application | Standard PCR, qPCR, RT-PCR |
| Unit Size | Each |
| Shipping Condition | Wet or Dry Ice |
| Storage Conditions | -20°C |
Applications
1. PCR, qPCR, and RT-PCR Optimization
- Essential cofactor for Taq DNA Polymerase and other polymerases.
- Helps fine-tune magnesium concentration in PCR buffer systems.
- Improves yield, specificity, and fidelity of amplified DNA fragments.
2. Molecular Biology & Genomic Research
- Used in gene expression studies, genotyping, and sequencing workflows.
- Supports nucleic acid amplification in clinical, forensic, and environmental DNA analysis.
- Ensures efficient amplification of low-abundance and GC-rich DNA templates.
3. Enzymatic Reactions & Biochemical Assays
- Enhances enzyme activity in DNA polymerization and restriction digestion assays.
- Used in reverse transcription and cDNA synthesis protocols.
Pack Size & Catalog Number
| Pack Size | Catalog Number (SKU) |
|---|---|
| 5 mL | AB0359 |
Storage & Handling
- Store at -20°C to maintain stability.
- Avoid repeated freeze-thaw cycles to preserve reagent integrity.
- Use sterile pipettes to prevent contamination during handling.
Summary
The Thermo Scientific™ 25 mM Magnesium Chloride (MgCl₂) Solution is an essential PCR reagent designed to optimize polymerase activity and enhance amplification efficiency. With its pre-diluted, ready-to-use format, it simplifies PCR setup and ensures high reproducibility in molecular biology applications.