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GoTaq® Probe 2-Step RT-qPCR System, 50 × 20µl RT reactions and 200 × 20µl qPCR reactions
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Specifications:
| Application | qPCR, Reverse Transcription | ||
| Storage Temperature | -20°C | ||
| Product Type | PCR Reagent | Forms | Liquid |
| Product Brand | Promega | ||
| Product Grade | Molecular Biology | ||
Two-Step Reverse Transcription and qPCR
The GoTaq® Probe 2-Step RT-qPCR System facilitates detection and relative quantification of RNA expression levels via a two-step RT-qPCR method using integrated components: GoScript™ Reverse Transcription System and GoTaq® Probe qPCR Master Mix.
The GoTaq® Probe qPCR Master Mix contains GoTaq® Hot Start Polymerase, MgCl2, dNTPs and a proprietary reaction buffer. The master mix does not contain a reference dye; a separate tube of CXR reference dye is included.
The GoScript™ Reverse Transcription System includes an optimized reaction buffer and M-MLV reverse transcriptase designed to enable efficient synthesis of first-strand cDNA in preparation for PCR amplification. The cDNA product can be added directly to downstream qPCR amplification reactions.
| Size | Cat.# | 10µl Reactions | 20µl Reactions |
|---|---|---|---|
| A6110 | 2ml | 50 × 20µl RT reactions and 400 × 10µl qPCR reactions | 50 × 20µl RT reactions and 200 × 20µl qPCR reactions |
Amplification curve for GAPDH detection from human pancreas RNA using the GoTaq® Probe 2-Step RT-qPCR System.
Suitable for Different Organisms and Sample Types
DNA/RNA isolated from the following organisms and sample types has been used with GoTaq® qPCR Systems. DNA/RNA from organisms and sample types not listed may also be compatible—please enquire with Promega technical support for additional compatibility.
Organism Types:
- Animal
- Bacteria
- Fungi
- Human
- Parasites
- Viruses
- Yeast
Sample Types:
- Blood
- Cells
- Feces
- FFPE
- Food
- Human Cerebrospinal Fluid (CSF)
- Plants
- Plasma
- Saliva
- Serum
- Sputum
- Swabs
- Tissue
- Urine