Applied Biosystems™ TaqMan™ Universal Extraction Control Organism

Lyophilized Bacillus atrophaeus Control for Nucleic Acid Extraction Process Validation

The TaqMan™ Universal Extraction Control Organism from Applied Biosystems™ is a lyophilized process control designed to monitor the efficiency of cell lysis and nucleic acid recovery during pathogen detection workflows. Supplied as three vials of Bacillus atrophaeus, this control organism is compatible with the proprietary TaqMan assay (ID Ba06596576_s1) and mimics difficult-to-lyse gram-positive organisms, making it ideal for rigorous process validation.

This control is intended for use with extraction protocols where accuracy, reproducibility, and contamination monitoring are critical. It may be run as a stand-alone control sample, incorporated into negative extraction controls, or spiked into test samples prior to extraction.

Key Features & Benefits

• ✅ Includes Bacillus atrophaeus – A tough-to-lyse gram-positive bacterium for realistic control
• ✅ Verifies Lysis and Extraction Efficiency – Monitors critical upstream steps in molecular workflows
• ✅ Flexible Use Options – Can be used as a standalone control, or added to test samples or negative controls
• ✅ High Copy Concentration – 1 × 10⁹ copies/vial ensures robust detection across multiple extractions
• ✅ Compatible with TaqMan Assay ID Ba06596576_s1 – Optimized for qPCR workflows
• ✅ For Research Use Only – Ideal for labs developing or validating pathogen detection assays

Technical Specifications

Application Workflow

1. Reconstitute each pellet in 200 µL 1X PBS (pH 7.4).
2. Spike 10 µL into the sample matrix (e.g., universal transport media).
3. Process alongside test samples through the full extraction and detection workflow.
4. Monitor recovery using TaqMan™ assay for Bacillus atrophaeus to verify extraction consistency.

Applications

🔬 Pathogen Detection Assay Validation – Use as a control to confirm nucleic acid recovery efficiency

🧪 Nucleic Acid Extraction QC – Monitor batch-to-batch consistency and reagent performance

🏥 Laboratory Method Development – Implement as a standard in assay verification protocols

🧬 Molecular Workflow Troubleshooting – Detect upstream inefficiencies or protocol failures

Ordering Information

The TaqMan™ Universal Extraction Control Organism offers a robust and realistic method to monitor extraction and lysis performance in molecular detection workflows. With compatibility across sample types and flexible usage in quality control, this control supports standardization and reliability in research-grade pathogen surveillance and assay development.