Gibco™ Terrific Broth, 1L

Gibco™ Terrific Broth is a ready-to-use liquid growth medium designed for the cultivation and maintenance of E. coli strains, especially in experiments where high plasmid DNA yield or high-density growth is required. This filter-sterilized broth is ideal for a variety of molecular biology applications, including protein expression, cloning, and cell culture expansion.

Terrific Broth is a nutrient-rich medium developed to significantly improve plasmid yield—4–7 times more DNA compared to LB medium when using pUC-based vectors. It is also highly effective for processing multiple clones from smaller culture volumes.

Key Features & Benefits:

1. Enhanced Plasmid DNA Yield:
   • Terrific Broth supports the growth of recombinant E. coli strains, producing 4–7 times more plasmid DNA compared to traditional LB medium, making it ideal for plasmid DNA preparation.
2. Nutrient-Rich Formula:
   • Contains higher concentrations of yeast extract compared to other growth media, providing additional nutrients for E. coli growth and ensuring higher bacterial density.
   • The combination of glycerol and high salt concentration improves buffering capacity and provides a stabilizing effect on recombinant proteins, making it perfect for protein expression experiments.
3. Ready-to-Use and Sterile:
   • The medium is pre-prepared, sterile-filtered, and ready for immediate use in the lab, reducing preparation time and the risk of contamination.
4. Improved Protein Expression:
   • The high glycerol content in Terrific Broth has a stabilizing effect on recombinant proteins, making it an excellent choice for protein expression experiments where stability and yield are crucial.
5. Environmental Considerations:
   • The bottle and cap are made from recyclable materials, such as HDPE and PETE, supporting sustainability in the laboratory.

Applications:

• Growth of E. coli strains for high plasmid DNA yield.
• Expression of recombinant proteins using bacterial cultures.
• Molecular cloning experiments involving E. coli.
• Protein expression studies that require stable, high-yield production of recombinant proteins.

Terrific Broth Formulation (per liter):

• 11.8 g Tryptone: Provides nitrogen, amino acids, and essential growth factors.
• 23.6 g Yeast Extract: Rich source of vitamins (including B-group vitamins) and peptides.
• 9.4 g Dipotassium Hydrogen Phosphate: Buffering agent to maintain pH stability.
• 2.2 g Potassium Dihydrogen Phosphate: Provides additional buffering capacity.
• 4 mL Glycerol (0.4%): Stabilizes recombinant proteins and enhances bacterial growth.

Specifications:

Storage:

• Store at room temperature.

Gibco™ Terrific Broth is an optimized, nutrient-rich medium perfect for high-efficiency cloning, protein expression, and plasmid DNA preparation. Its ability to provide high plasmid yield, coupled with its nutrient-packed formulation, makes it ideal for molecular biology, biotechnology, and genetic engineering applications. Whether you're working on protein expression or cloning experiments, Terrific Broth offers enhanced efficiency, yielding high-quality results every time.