Greiner 96-well plates, PP, Flat Bottom, Chimney Style, Black

Greiner 96-Well Plates (PP, Flat Bottom, Chimney Style, Black) are high-quality, chemically resistant microplates designed for fluorescence and luminescence assays, high-throughput screening, and compound storage. These plates feature a flat-bottom, chimney-well design, which minimizes well-to-well crosstalk and evaporation, ensuring high accuracy and reproducibility in sensitive biochemical assays.

Made from polypropylene (PP), these plates offer superior chemical resistance and heat stability, making them ideal for long-term liquid storage, compound libraries, and drug discovery applications. Their black color significantly reduces background fluorescence, improving signal detection in fluorescence and luminescence-based experiments.

Key Features & Benefits

1. High Chemical & Heat Resistance
   • Made from polypropylene (PP) for superior chemical compatibility.
   • Withstands DMSO, organic solvents, and temperature variations.
2. Chimney-Style Wells for Reduced Evaporation
   • Individual chimney-like wells minimize edge effects and cross-contamination.
   • Supports long-term sample integrity in storage applications.
3. Optimized for Fluorescence & Luminescence Assays
   • Black polypropylene reduces background fluorescence and light scatter.
   • Ensures high signal-to-noise ratios in fluorescence detection assays.
4. Standard Microplate Footprint & High-Throughput Compatibility
   • ANSI/SLAS-compliant design ensures compatibility with automated liquid handling systems.
   • Suitable for robotic sample processing, high-throughput screening (HTS), and plate readers.
5. Contaminant-Free for Molecular Biology & Drug Screening
   • Certified DNase/RNase-free, human DNA-free, and non-pyrogenic (<0.06 EU/mL).
   • Ideal for cell-based assays, compound storage, and bioassay development.

Product Specifications

Use Cases & Applications

1. Fluorescence & Luminescence-Based Assays

• Optimized for Fluorescence-Based Detection Assays
  • Used in fluorescence resonance energy transfer (FRET), GFP-based assays, and protein binding studies.
  • Ensures minimal background fluorescence for highly sensitive signal detection.
• Enhanced Sensitivity in Luminescence-Based Assays
  • Supports bioluminescent and chemiluminescent assays with high signal clarity.
  • Reduces light scatter and crosstalk between wells.

2. High-Throughput Screening (HTS) & Drug Discovery

• Compatible with Automated Liquid Handling Systems
  • Designed for robotic pipetting, liquid dispensing, and compound screening.
  • Meets ANSI/SLAS microplate standards for seamless integration with automation systems.
• Chemical-Resistant for Compound Storage & Screening
  • Suitable for long-term storage of drug compounds and reagents.
  • Withstands DMSO and other organic solvents without degradation.

3. ELISA, Immunoassays & Biochemical Studies

• Flat-Bottom Wells for High-Quality Optical Readouts
  • Ideal for homogeneous ELISA reactions and absorbance-based assays.
  • Ensures consistent and uniform well-to-well readings.
• Minimized Well-to-Well Crosstalk in Enzyme-Linked Immunoassays
  • Supports chemiluminescent, fluorescent, and chromogenic ELISA applications.
  • Reduces signal interference between wells.

4. Cell-Based Assays & Imaging Applications

• Ideal for Cell Culture & Cytotoxicity Testing
  • Black polypropylene reduces photobleaching effects in live-cell fluorescence imaging.
  • Compatible with automated cell imaging and high-content screening platforms.
• Microplate-Compatible for Fluorescence Microscopy
  • Used for fluorescent protein expression analysis and cellular metabolism assays.
  • Ensures high-quality imaging with minimal background interference.

5. Molecular Biology & Genomics

• PCR & qPCR Sample Handling
  • Compatible with DNA/RNA extraction and nucleic acid quantification workflows.
  • Chemically inert for stable sample storage.
• Protein & Enzyme Studies
  • Suitable for enzyme assays, binding kinetics studies, and high-throughput screening (HTS) assays.
  • Ensures reproducible assay performance with low background interference.

Usage & Handling Instructions

Plate Handling & Sample Preparation

1. Ensure Compatibility with Detection Instruments:
   • Use fluorescence, luminescence, or absorbance-compatible plate readers.
   • Check wavelength compatibility for optimal signal detection.
2. Pipetting & Liquid Handling:
   • Use multi-channel pipettes or automated liquid handlers for even distribution.
   • Avoid bubble formation to prevent signal interference in spectroscopic assays.
3. Incubation & Storage:
   • Seal plates with adhesive film or breathable seals for long-term assays.
   • Store plates at room temperature or 4°C, depending on sample requirements.
4. Fluorescence & Luminescence Detection:
   • Minimize exposure to ambient light to reduce photobleaching effects.
   • Use black plates for low-background readings and high signal contrast.

Storage & Handling Instructions

• Storage Conditions:
  • Store at room temperature in a dry, dust-free environment.
  • Protect from prolonged exposure to light and heat.
• Handling Precautions:
  • Use gloves to prevent contamination when handling plates.
  • Ensure plates are stacked properly to avoid warping or physical damage.
• Disposal Guidelines:
  • Dispose of plates as per laboratory biohazard or chemical waste disposal protocols.
  • If used with hazardous materials, autoclave before disposal.

Why Choose Greiner 96-Well Plates (655209)?

✅ Black polypropylene material for superior fluorescence & luminescence assays

✅ Chimney-style wells reduce evaporation and cross-contamination

✅ Flat-bottom design ensures uniform sample distribution & optical clarity

✅ Solvent-resistant for use with DMSO & organic compounds

✅ Compatible with robotic liquid handlers & high-throughput screening systems

Greiner 96-Well Plates (655209) are a premium choice for fluorescence, luminescence, and high-throughput assays, ensuring precise, reproducible, and contamination-free results in molecular biology, drug discovery, and biochemical research.