QIAamp® DNA Microbiome Kit, 50 Preps

The QIAamp DNA Microbiome Kit is a specialized solution designed for the efficient purification and enrichment of bacterial microbiome DNA from mixed biological samples such as swabs and body fluids. By depleting host DNA and optimizing bacterial DNA recovery, the kit supports accurate and high-resolution microbiome analysis, including 16S rDNA sequencing and metagenomic shotgun studies.

Key Features

1. Effective Host DNA Depletion:
   • Achieves over 90% host DNA removal, reducing human reads in metagenomic studies to less than 5%.
   • Significantly improves bacterial DNA coverage for sensitive downstream analyses.
2. Optimized Lysis Protocols:
   • Enzymatic host DNA degradation prior to bacterial cell lysis.
   • Combines mechanical and chemical lysis for unbiased disruption of diverse bacterial cell walls.
3. Ultra-Clean Spin Columns:
   • Utilizes proprietary Ultra Clean Production (UCP) columns to minimize contamination risks.
4. Reliable Performance:
   • Enhances the amplification and sequencing of 16S rDNA, delivering accurate microbial community compositions.
   • Consistent and unbiased bacterial DNA recovery from mixed samples.
5. Rapid and Efficient Workflow:
   • Simple, spin-column-based protocol ensures reliable DNA extraction with minimal processing bias.

Kit Components

Applications

The kit is designed for use in microbiome research and supports applications such as:

1. Whole Metagenome Sequencing:
   • Enriches microbial DNA for comprehensive analysis.
2. 16S rDNA Sequencing:
   • Enhances sensitivity for detecting microbial community compositions.
3. Metagenomic Shotgun Sequencing:
   • Reduces host contamination for deeper microbial insights.

Performance Highlights

1. Host DNA Removal:
   • Reduces human-derived reads to less than 5%, compared to 90% without host DNA removal.
   • Allows for greater focus on bacterial genomic content.
2. Microbial DNA Recovery:
   • High bacterial species coverage demonstrated in spiked buccal swab samples.
   • Improved amplification and sequencing accuracy for microbiome studies.
3. Enhanced 16S rDNA Analysis:
   • Optimized for efficient amplification of the V4 region in bacterial DNA.

Procedure

The QIAamp DNA Microbiome Kit uses a spin-column technology with an integrated host DNA removal protocol:

1. Host Lysis:
   • Gently lyses host cells to release host DNA while preserving bacterial cells.
2. Host DNA Depletion:
   • Enzymatically degrades released host DNA.
3. Bacterial Lysis:
   • Mechanical and chemical lysis ensures comprehensive disruption of bacterial cells.
4. DNA Purification:
   • Released bacterial DNA binds to the silica membrane for purification.
5. Elution:
   • High-purity bacterial DNA is eluted for downstream applications.

Specifications

Advantages

1. High Sensitivity:
   • Superior host DNA removal ensures greater microbial DNA recovery.
2. Unbiased Results:
   • Reduces sample preparation bias for accurate microbial community representation.
3. Ease of Use:
   • Simple spin-column protocol with pre-packaged reagents.
4. Compatibility:
   • Suitable for a wide range of downstream molecular biology applications, including NGS and PCR.

Why Choose the QIAamp DNA Microbiome Kit?

• Reliable Host DNA Depletion: Minimize contamination from host DNA for cleaner, more accurate microbiome data.
• Optimized Microbial Enrichment: High bacterial DNA recovery for enhanced sequencing performance.
• Proven Performance: Validated for whole genome sequencing, 16S rDNA analysis, and metagenomic studies.

The QIAamp DNA Microbiome Kit is an essential tool for microbiome researchers, enabling high-quality DNA preparation for advanced molecular and genomic studies.