Applied Biosystems™ PrepFiler Express BTA™ Forensic DNA Extraction Kit, 52 Preps

The PrepFiler™ Express BTA Forensic DNA Extraction Kit is a fully automated, magnetic bead–based solution designed for high-efficiency DNA purification from challenging forensic samples. Engineered for use with the AutoMate Express™ Forensic DNA Extraction System, this kit delivers consistent, high-quality genomic DNA suitable for demanding downstream applications such as STR analysis and quantitative PCR.

Specifically optimized for difficult substrates including bones, teeth, cigarette butts, and adhesive-based samples, the PrepFiler™ Express BTA kit enhances DNA recovery while minimizing PCR inhibitors. Its integrated workflow, featuring pre-filled cartridges and pre-programmed protocols, ensures rapid, reproducible, and contamination-controlled DNA extraction.

Key Features & Benefits

Optimized for Challenging Forensic Samples

• Designed for difficult matrices such as:
  • Bones and teeth
  • Cigarette butts and tape lifts
  • Degraded and low-template samples
• Maximizes DNA recovery where traditional methods may fail

Fully Automated Workflow

• Compatible with AutoMate Express™ system for streamlined processing
• Processes 1 to 13 samples per run
• Pre-validated protocols reduce operator variability

High Purity DNA for Downstream Applications

• Produces inhibitor-free DNA suitable for:
  • STR profiling
  • Real-time quantitative PCR (qPCR)
• Improves genotyping success rates and profile quality

Innovative LySep Column Technology

• Enables rapid separation of substrate from lysate
• Eliminates manual transfer steps
• Reduces risk of cross-contamination and sample mix-ups

Closed System for Enhanced Reliability

• Minimizes contamination and transposition errors
• Ensures reproducibility and traceability in forensic workflows

Ready-to-Use Cartridge System

• Pre-filled cartridges simplify setup and reduce preparation time
• Enables faster turnaround for forensic casework

Applications

• Forensic DNA extraction from challenging and degraded samples
• Criminal investigations and forensic casework
• Human identification and DNA databasing
• Paternity and kinship analysis
• STR profiling and qPCR-based workflows

Technical Specifications

Kit Contents

• PrepFiler BTA Lysis Buffer
• PrepFiler LySep Columns
• AutoMate Express tips and tip holder
• PrepFiler Bone and Tooth Lysate Tubes
• Proteinase K
• PrepFiler Express pre-filled cartridges
• Sample tubes
• Elution tubes

Unique Advantages

• Specialized chemistry for bone, teeth, and adhesive samples
• Automated workflow reduces hands-on time and human error
• Integrated system ensures consistent and reproducible results
• LySep technology simplifies lysis and improves workflow efficiency
• High-quality DNA output for reliable STR and qPCR analysis

The PrepFiler™ Express BTA Forensic DNA Extraction Kit is a powerful and reliable solution for forensic laboratories requiring automated, high-quality DNA extraction from challenging samples. With advanced chemistry, innovative sample processing technology, and seamless integration with the AutoMate Express™ system, it enables laboratories to achieve maximum DNA recovery, improved data quality, and operational efficiency in forensic workflows.