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Applied Biosystems™ AmpFLSTR™ MiniFiler™ PCR Amplification Kit, 100 Rxn
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Specifications:
| Application | qPCR, Forensics Human identification | ||
| Storage Temperature | -20°C, 2-8°C | ||
| Product Type | PCR Amplification Kit | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Applied Biosystems™ | ||
| Product Grade | Molecular Biology | ||
The AmpFLSTR™ MiniFiler™ PCR Amplification Kit is a specialized miniSTR multiplex system designed to enhance DNA profiling success from highly degraded and compromised forensic samples. As the first commercially available miniSTR kit, MiniFiler™ enables forensic laboratories to recover genetic information from samples that would otherwise yield limited or no usable data.
By targeting short amplicon STR loci (<270 bp), this kit significantly improves amplification efficiency in degraded DNA, making it an essential tool for cold case investigations, trace evidence analysis, and challenging forensic workflows.
Key Features
Optimized for Degraded & Inhibited Samples
- Short amplicon design (<270 bp) ensures high success rates with degraded DNA
- Effective performance in the presence of common inhibitors such as heme and humic acid
- Ideal for low-quality, aged, or environmentally exposed samples
Targeted STR Recovery
- Amplifies the largest and most difficult loci from standard STR kits (e.g., Identifiler™, SGM Plus)
- Enables recovery of loci that may drop out in conventional STR amplification
- Functions as a complementary tool to full STR profiling kits
Advanced 5-Dye Technology
- Utilizes FAM, VIC, NED, PET, and LIZ dyes
- Expanded spectral range (up to 660 nm) for improved color separation
- Supports high-throughput multiplexing and simplified workflow
Integrated & Validated Workflow
- Fully compatible with Applied Biosystems™ instruments and software
- Part of a comprehensively validated STR-based human identification system
- Developed in accordance with global forensic standards (e.g., SWGDAM, CODIS, ENFSI, Interpol)
High Sensitivity & Reliability
- Incorporates AmpliTaq Gold™ DNA Polymerase for robust amplification
- Provides clean, interpretable profiles even from difficult samples
- Enables re-analysis of cold cases and previously inconclusive evidence
Applications
- Forensic DNA analysis of degraded samples
- Cold case re-investigation and DNA recovery
- Trace and low-template DNA profiling
- Complementary STR analysis for allele dropout recovery
- Criminal investigations and legal evidence processing
Technical Specifications
| Parameter | Description |
|---|---|
| Product Name | AmpFLSTR™ MiniFiler™ PCR Amplification Kit |
| Brand | Applied Biosystems™ |
| Technology | STR (Short Tandem Repeat) Analysis (miniSTR) |
| Detection Method | Capillary Electrophoresis (Fluorescence-based) |
| Dye Set | FAM, VIC, NED, PET, LIZ |
| Polymerase | AmpliTaq Gold™ DNA Polymerase |
| Amplicon Size | <270 bp |
| No. of Reactions | 100 |
| Reaction Volume | 25 μL |
| Sample Type | Genomic DNA, highly degraded forensic samples |
| Format | Kit (Master Mix / SuperMix) |
| Storage Conditions | -20°C (long-term), 2–8°C after opening |
| Shipping Condition | Dry Ice |
Kit Contents & Storage
- AmpFLSTR™ MiniFiler™ Primer Set
- AmpFLSTR™ MiniFiler™ Master Mix
- AmpFLSTR™ MiniFiler™ Allelic Ladder
- Control DNA (Male 007)
Storage:
- Core reagents: Store at -20°C, then 2–8°C after initial use
- Control DNA: Store at 2–8°C
The AmpFLSTR™ MiniFiler™ PCR Amplification Kit is a critical solution for forensic laboratories dealing with challenging, degraded, or inhibited DNA samples. By leveraging miniSTR technology, short amplicon design, and robust chemistry, it significantly improves the likelihood of obtaining usable genetic profiles where conventional methods fail. As a complementary tool within the forensic DNA workflow, MiniFiler™ enhances data recovery, supports cold case resolution, and strengthens the reliability of forensic evidence analysis.