Thermo Scientific™ Invitrogen™ Proteinase K Solution, 5 mL

Proteinase K from the fungus Engyodontium album is a non-specific serine protease used for the general digestion of proteins. This enzyme is widely used in DNA and RNA extraction procedures, as it efficiently removes endogenous nucleases, which can degrade nucleic acids during preparation.

The enzyme retains high activity across a wide pH range (with optimal activity between 6.5 and 9.5) and is effective under denaturing conditions, such as the presence of SDS, urea, and metal chelating agents like EDTA. It also works well at relatively high temperatures, with an optimum digestion temperature of 65°C. The versatility of Proteinase K makes it ideal for various biochemical and molecular biology applications, including genetic studies, chromatin biology, and in situ hybridization.

Key Features:

1. Non-Specific Protease:
   • Proteinase K is a serine protease that digests proteins without specificity, making it ideal for general protein removal and nucleic acid preservation.
2. Wide Activity Range:
   • Active across a wide pH range (optimal between 6.5–9.5) and can function under denaturing conditions such as SDS, urea, and EDTA.
3. High-Temperature Stability:
   • Active at elevated temperatures, with an optimum temperature for digestion at 65°C.
4. DNase/RNase-Free:
   • Purified to be DNase-free and RNase-free, ensuring no degradation of DNA or RNA during extraction.
5. Applications:
   • Used for protein digestion, DNA extraction, RNA extraction, and in situ hybridization.
6. Purity Testing:
   • Tested for absence of RNase activity and endodeoxyribonuclease and exodeoxyribonuclease activities, ensuring a high-quality enzyme for laboratory procedures.
7. Ease of Use:
   • Supplied as a liquid solution for easy handling and dilution.

Specifications:

Applications:

1. Protein Digestion:
   • Ideal for removing proteins during nucleic acid extraction processes, especially in genomic DNA and RNA extractions.
2. DNA Extraction:
   • Used in DNA extraction protocols to remove proteins that can interfere with downstream applications like PCR or sequencing.
3. RNA Extraction:
   • Effectively removes endogenous nucleases during RNA extraction, preserving the integrity of RNA for RT-PCR or gene expression studies.
4. In Situ Hybridization:
   • Useful in tissue section preparation for in situ hybridization studies, removing contaminants that might otherwise affect the probe-target interaction.
5. Protein Removal in Biochemical Assays:
   • Efficient for protein removal in preparation for proteomic analysis or other biochemical studies requiring clean nucleic acid samples.

Invitrogen™ Proteinase K Solution is an essential enzyme for protein digestion during DNA and RNA extraction protocols, particularly in chromatin biology and in situ hybridization applications. Its broad activity range, high-temperature stability, and high purity make it a reliable reagent in various molecular biology procedures.