VWR® BDH Folin-Ciocalteu'S Reagent For Analysis Of Phenols, 500Ml

VWR® Folin-Ciocalteu’s Reagent is a widely used colorimetric reagent for the quantification of total phenolic content in biological, food, and environmental samples. This reagent, also known as the Folin-Denis or Folin-Ciocalteu assay reagent, is based on a phosphomolybdate-phosphotungstate complex, which reacts with phenolic compounds to produce a measurable blue chromophore.

The Folin-Ciocalteu method is a reliable and sensitive technique used in food science, natural product chemistry, environmental analysis, and pharmaceutical research to determine total phenolic content (TPC) in plant extracts, beverages, and other biological samples.

This VWR®-certified Folin-Ciocalteu reagent ensures high accuracy and reproducibility, making it suitable for both routine laboratory assays and advanced research applications.

Key Features & Benefits

1. High Sensitivity for Phenolic Compound Detection
   • Detects phenols, polyphenols, flavonoids, tannins, and other reducing agents.
   • Produces a blue color with a peak absorbance at 750-765 nm for spectrophotometric analysis.
2. Reliable & Reproducible Quantification Method
   • Compatible with UV-Vis spectrophotometers and plate readers.
   • Used in both manual and automated total phenolic content (TPC) assays.
3. Versatile Applications in Food, Biological, & Environmental Analysis
   • Measures antioxidant capacity in foods and beverages.
   • Quantifies phenolic content in plant extracts, pharmaceuticals, and environmental samples.
4. Stable & Ready-to-Use Reagent
   • Available in liquid form for immediate use in assays.
   • Long shelf life when stored under proper conditions.
5. Meets Laboratory & Research Standards
   • Ensures accurate and reproducible results in biochemical, agricultural, and industrial studies.
   • Compatible with standard protocols for total phenol quantification.

Product Specifications

Use Cases & Applications

1. Food & Beverage Analysis

• Determination of Total Phenolic Content in Food & Drinks:
  • Used in wine, tea, coffee, fruit juices, and plant extracts to measure antioxidant levels.
  • Helps assess nutritional value and quality control in food products.
• Quantification of Antioxidants & Polyphenols in Nutraceuticals:
  • Evaluates antioxidant capacity in dietary supplements and functional foods.
  • Supports standardization of plant-based health products.

2. Pharmaceutical & Natural Product Research

• Analysis of Plant-Derived Medicines & Herbal Extracts:
  • Measures bioactive compounds in herbal medicines and essential oils.
  • Used for quality control in pharmaceutical formulations.
• Measurement of Phenolic Antioxidants in Drug Discovery:
  • Assists in screening plant extracts for antioxidant activity.
  • Supports drug formulation studies related to oxidative stress.

3. Environmental & Agricultural Research

• Total Phenolic Content in Soil & Water Samples:
  • Used in soil and water quality monitoring to detect phenolic pollutants.
  • Supports ecotoxicological studies on organic contamination.
• Characterization of Plant Metabolites & Secondary Compounds:
  • Analyzes phenolic compounds in agricultural crops and medicinal plants.
  • Helps improve breeding strategies for high-antioxidant crop varieties.

4. Biochemical & Analytical Laboratory Applications

• Antioxidant Activity Assays in Biological Samples:
  • Measures polyphenolic compounds in plasma, serum, and cell lysates.
  • Used in studies on oxidative stress, disease prevention, and metabolic pathways.
• Total Phenolic Quantification for Clinical & Nutritional Research:
  • Supports human health studies by evaluating dietary polyphenol intake.
  • Helps correlate phenolic content with disease prevention mechanisms.

Folin-Ciocalteu Assay Procedure Overview

Reagent Preparation & Standard Curve Development:

1. Prepare Standard Solutions (Gallic Acid or Equivalent Phenolic Standard).
2. Dilute the Folin-Ciocalteu’s Reagent with deionized water (typically 1:10 ratio).
3. Prepare the Calibration Curve using absorbance values at 750-765 nm.

Sample Analysis:

1. Mix the Sample with Folin-Ciocalteu’s Reagent in a test tube.
2. Add Sodium Carbonate Solution to initiate the colorimetric reaction.
3. Incubate for 30-60 minutes at room temperature.
4. Measure Absorbance at 750-765 nm using a spectrophotometer.

Storage & Handling Instructions

• Storage Conditions:
  • Store at 2-8°C in an airtight container to prevent contamination.
  • Keep away from direct sunlight and strong oxidizers.
• Handling Precautions:
  • Use protective gloves, safety goggles, and lab coats when handling.
  • Avoid ingestion and skin contact, as reagent is acidic.
  • Work in a well-ventilated area or fume hood.
• Disposal Guidelines:
  • Dispose of used reagent according to local chemical waste regulations.
  • Neutralize acidic waste before disposal.

Why Choose VWR® Folin-Ciocalteu’s Reagent?

✅ Reliable & highly sensitive reagent for total phenolic quantification

✅ Optimized for food, pharmaceutical, biochemical, and environmental applications

✅ Consistent & reproducible results with spectrophotometric detection

✅ Ready-to-use liquid formulation for convenience

✅ Trusted for quality control, research, and analytical chemistry

VWR® Folin-Ciocalteu’s Reagent is a high-quality analytical solution for phenolic compound quantification, making it a valuable tool for researchers, food scientists, pharmaceutical developers, and environmental analysts.