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Thermo Scientific™ Triton™ X-114 Surfact-Amps™ Detergent Solution, 6 x 10 mL
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Specifications:
| Application | molecular biology | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Molecular Biology Reagent | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, thermo Scientific | ||
| Product Grade | Molecular Biology | ||
Stabilized, High-Purity Nonionic Surfactant for Protein Extraction and Phase Separation Applications
Thermo Scientific™ Triton™ X-114 Surfact-Amps™ Detergent Solution is a pre-formulated, ultrapure 10% (w/v) aqueous solution of Triton™ X-114, a nonionic polyoxyethylene surfactant ideal for cell lysis, membrane protein extraction, and aqueous phase separation techniques. This highly purified detergent is packaged in glass ampules under inert nitrogen gas, preventing oxidation and degradation during storage and ensuring reproducibility in sensitive biochemical workflows.
Triton X-114 is known for its low cloud point (~23 °C), which enables temperature-induced phase separation—an important feature for the selective partitioning of hydrophilic and hydrophobic proteins, especially in proteomics and membrane biology.
Key Features & Benefits
- Ready-to-Use Solution – Pre-diluted to 10% (w/v) in ultrapure water for immediate use
- Phase Separation Capable – Cloud point of 23 °C enables extraction of membrane proteins
- Stabilized Packaging – Supplied in nitrogen-sealed glass ampules to prevent peroxide accumulation
- Exceptionally Low Contaminants – ≤1.0 µeq/mL peroxides and carbonyls for enhanced purity
- Reliable for Sensitive Applications – Ideal for protein biochemistry, cell fractionation, and extraction of detergent-sensitive targets
Applications
- Cell Lysis – Disrupts cell membranes gently while maintaining protein structure
- Membrane Protein Solubilization – Preferred for isolating transmembrane and hydrophobic proteins
- Phase Separation Techniques – Utilized to partition cytosolic vs. membrane-associated proteins
- Protein Extraction in Proteomics – Compatible with downstream mass spectrometry and Western blotting
- Microsome and Organelle Isolation – Facilitates separation of subcellular components by detergent phase partitioning
Technical Specifications
| Attribute | Specification |
|---|---|
| Product Name | Triton™ X-114 Surfact-Amps™ Detergent Solution |
| Catalog Number | 28332 |
| Concentration | 10.0 ± 1.0% (w/v) |
| Form | Liquid (aqueous solution) |
| Volume | 6 × 10 mL ampules |
| Packaging | Glass ampules, nitrogen-sealed |
| Oxidants | ≤1.0 μeq/mL |
| Carbonyls | ≤1.0 μeq/mL |
| Suspended Solids | Must not exceed residue reference |
| Visual Appearance | Slightly hazy to cloudy, colorless; may form visible layers |
| Detergent Type | Nonionic polyoxyethylene surfactant |
| Molecular Weight | ~537 g/mol |
| Critical Micelle Concentration (CMC) | ~0.21 mM (0.0113% w/v) |
| Cloud Point | ~23 °C |
| Dialyzable | No |
| Storage Conditions | 20°C to 25°C, away from direct sunlight |
| Regulatory Use | For Research Use Only |
Handling Note
- Consider using Thermo Scientific™ Ampule Breakers for safe handling and opening of glass ampules.
Thermo Scientific™ Triton™ X-114 Surfact-Amps™ Detergent Solution is a reliable, ultrapure, and stabilized surfactant solution that facilitates membrane protein extraction and phase separation-based purification workflows. Its precisely formulated 10% solution and nitrogen-sealed packaging make it a critical reagent for researchers in molecular biology, proteomics, and biochemical analysis.