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Thermo Scientific™ Pierce™ Luciferase Cell Lysis Buffer (2X), 250mL
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Specifications:
| Application | Fluorescence-Based Assay | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Buffer | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Thermo Scientific™ Pierce™ Luciferase Cell Lysis Buffer (2X) is a ready-to-use, high-performance reagent for efficient lysis of adherent and suspension mammalian cells, enabling high-yield lysate preparation for luciferase reporter assays.
Validated across a wide range of mammalian cell lines, including COS-7, NIH3T3, Jurkat, Hepa 1-6, 293, CHO, MDA-MB-231, and FM2, this buffer ensures rapid and complete cell disruption while maintaining compatibility with protein quantification assays such as the Pierce™ BCA Protein Assay and Coomassie Plus (Bradford) Assay.
Designed for optimal performance with Pierce Luciferase Assay Kits, it provides consistent results for promoter activity studies, gene expression analysis, and transfection efficiency evaluation.
Key Features
- Compatible with All Pierce Luciferase Reporter Assays – Ensures maximum signal and reproducibility.
- Rapid & Efficient Lysis – Delivers complete cell disruption within minutes.
- Broad Cell Line Compatibility – Proven with multiple adherent and suspension cell types.
- Protein Assay Compatible – Works with Coomassie Plus (Bradford) and BCA assays for protein quantification.
- Convenient Ready-to-Use Format – No additional preparation required.
Applications
- Luciferase Reporter Gene Assays – Quantify promoter activity and transcriptional regulation.
- Transfection Studies – Assess efficiency of DNA, RNA, or CRISPR/Cas9 delivery.
- Gene Expression Analysis – Measure reporter activity in various experimental systems.
- Protein Quantification – Prepare lysates for downstream protein analysis.
Technical Specifications
| Parameter | Specification |
|---|---|
| Format | Liquid |
| Concentration | 2X |
| Volume | 250 mL |
| Type | Cell Lysis Buffer |
| Compatible Assays | Pierce Luciferase Reporter Kits, Coomassie Plus (Bradford), BCA |
| Validated Cell Lines | COS-7, NIH3T3, Jurkat, Hepa 1-6, 293, CHO, MDA-MB-231, FM2 |
| Storage | Room Temperature |
| Unit Size | Each |
The Thermo Scientific™ Pierce™ Luciferase Cell Lysis Buffer (2X) provides fast, efficient, and reproducible cell lysis for luciferase-based assays while maintaining compatibility with downstream protein quantification methods. Its broad cell line compatibility and ready-to-use formulation make it an essential reagent for molecular biology and gene expression research.