Pierce™ Firefly Signal Enhancer (100X), 0.5mL

The Thermo Scientific™ Pierce™ Firefly Signal Enhancer (100X) is a powerful additive designed to maximize the sensitivity and stability of firefly luciferase bioluminescence assays. Specially formulated for use with the Pierce™ Firefly Luciferase Flash Assay Kit (Cat. Nos. 16174, 16175) and the Pierce™ Firefly Luciferase Glow Assay Kit (Cat. Nos. 16176, 16177), this enhancer significantly improves signal intensity and signal duration.

When paired with the Firefly Flash Assay, the enhancer delivers up to a five-fold increase in signal brightness. When used with the Firefly Glow Assay, it enhances signal strength and extends luminescence stability up to two-fold, making it particularly beneficial for longer reading times or high-throughput formats.

Key Features

• Up to 5× Brighter Flash Signal – Maximizes sensitivity for low-level luciferase expression.
• Up to 2× Stronger Glow Signal – Increases intensity and extends signal stability.
• Easy-to-Use – Supplied as a convenient 100X concentrate.
• Versatile – Compatible with both flash and glow firefly luciferase assay formats.
• Enhanced Assay Performance – Ideal for promoter studies, screening assays, and multiplexed reporter systems.

Applications

• Gene Expression Studies – Detect low-abundance promoter-driven luciferase activity.
• Drug and Compound Screening – Improve assay signal for better hit detection.
• RNAi and miRNA Studies – Boost detection sensitivity in reporter assays.
• Signal Transduction Analysis – Enhance reporter signal in pathway studies.
• High-Throughput Screening – Maintain strong, stable signal for automated plate reading.

Technical Specifications

The Pierce™ Firefly Signal Enhancer is an essential upgrade for firefly luciferase assays, delivering stronger, longer-lasting signals for superior sensitivity and reproducibility. Whether for basic research or high-throughput screening, it ensures optimal assay performance when every photon counts.