Invitrogen™ S.O.C. Medium, 10 mL

S.O.C. Medium (Super Optimal broth with Catabolite repression) is a specialized growth medium used in the final step of bacterial cell transformation to achieve maximal transformation efficiency of E. coli cells. This medium is designed to optimize the recovery of transformed cells after the stress induced by the transformation process, resulting in 2–3 fold higher efficiency compared to recovery in LB medium.

The medium contains glucose to achieve catabolite repression, creating optimal metabolic conditions for E. coli growth. It also provides high concentrations of nutrients to help the cells recover and proliferate rapidly.

Key Features & Benefits:

1. Maximized Transformation Efficiency:
   • S.O.C. Medium enhances the recovery of transformed cells, significantly increasing the transformation efficiency of E. coli cells after chemical transformation.
   • The presence of glucose creates optimal conditions for recovery, resulting in 2–3 fold higher efficiency compared to using LB medium.
2. Rich Nutrient Content:
   • The high nutrient content of S.O.C. Medium supports bacterial growth and recovery, helping the cells recover from the stresses of the transformation process.
3. Convenient, Ready-to-Use Format:
   • S.O.C. Medium is supplied in 10 x 10 mL bottles, ready-to-use, eliminating the need for preparation and reducing the risk of contamination.
4. Optimized for E. coli Recovery:
   • The medium formulation, which includes 10 mM MgCl₂ and 20 mM glucose, is based on the protocol developed by Hanahan (1983), ensuring maximum recovery of E. coli cells.
5. Quality Testing:
   • Each lot of S.O.C. Medium is quality tested to ensure it meets current approved product specifications, including tests for pH, osmolality, sterility, and transformation efficiency using TOP10 E. coli.

Applications:

• Bacterial Transformation: Essential for recovering E. coli cells after transformation, ensuring efficient plasmid uptake and high transformation success.
• Maximal E. coli Recovery: Ideal for cloning applications where E. coli strains such as TOP10 or DH5α are transformed with plasmids and need to recover rapidly.
• High-Efficiency Transformation: Optimized for protocols requiring high-efficiency transformation, such as genetic engineering, recombinant DNA research, and protein expression.
• Cloning and Gene Expression Studies: Used in cloning experiments, cDNA library construction, and gene expression studies.

S.O.C. Medium Formulation:

• 2% Tryptone: Provides nitrogen, amino acids, and vitamins for bacterial growth.
• 0.5% Yeast Extract: Supplies essential B-vitamins and trace elements for cell metabolism.
• 10 mM NaCl: Maintains osmotic balance and supports nutrient transport.
• 2.5 mM KCl: Supplies potassium ions for cellular function.
• 10 mM MgCl₂: Essential for transformational recovery, stabilizing DNA during transformation.
• 10 mM MgSO₄: Supports enzymatic reactions during cell recovery.
• 20 mM Glucose: Enhances recovery and metabolic activity by promoting catabolite repression.

Specifications:

Contents:

• 10 x 10 mL bottles of S.O.C. Medium
• S.O.C. medium ready-to-use for immediate bacterial recovery after transformation.

Storage:

• Store at room temperature. No refrigeration is required for short-term storage.

Thermo Scientific™ S.O.C. Medium is an essential component for ensuring maximum transformation efficiency of E. coli cells following chemical transformation. With its high nutrient content and optimized formulation, S.O.C. Medium ensures that your E. coli cells recover efficiently and are ready for further analysis or cloning applications. Its convenient ready-to-use format saves time and simplifies workflows in molecular biology and biotechnology labs.