Thermo Scientific™ Gibco™ Trypan Blue Solution, 0.4%

Gibco™ Trypan Blue Solution, 0.4% is a cell viability stain commonly used to assess cell health via the dye exclusion test. This test involves the principle that viable cells do not absorb impermeable dyes like Trypan Blue, whereas dead cells become permeable and take up the dye. This is a simple and effective method to quickly determine cell viability during cell culture work.

The Trypan Blue Solution is particularly useful for cell counting and viability testing when using a hemocytometer or other cell counting methods. It is commonly used in routine subculturing or any time the viability of cells needs to be determined rapidly and accurately.

The product is sterile-filtered and manufactured under cGMP standards at a facility certified with ISO 13485. The solution is ready-to-use, non-toxic, and can be used for various mammalian cell types.

Key Features:

1. Cell Viability Testing:
   • Trypan Blue Solution is used for assessing cell viability in mammalian cell cultures using the dye exclusion method.
2. Non-Toxic and Easy to Use:
   • Trypan Blue is safe for cell viability testing and simple to use for rapid analysis. The solution is pre-prepared for immediate use.
3. Sterile-Filtered:
   • The solution is sterile-filtered, ensuring no contamination when testing cells.
4. cGMP Manufacturing:
   • Manufactured under cGMP-compliant conditions, ensuring high-quality standards and consistency.
5. Wide Application:
   • Commonly used in cell counting applications with a hemocytometer or other automated cell counting methods.
6. Colorimetric Detection:
   • Colorimetric detection of viable and non-viable cells. Viable cells remain colorless, while dead cells turn blue due to dye uptake.
7. Shelf Life:
   • The solution has a 36-month shelf life when stored at room temperature (15 – 30°C).
8. Convenient Packaging:
   • 100 mL quantity, perfect for routine use in laboratories.

Specifications:

Applications:

1. Cell Counting:
   • Widely used in cell viability testing and cell counting in mammalian cell cultures. This helps assess the health of cells and determine the concentration of viable cells in a given sample.
2. Routine Cell Culture:
   • Essential for regular subculturing of cell lines and for checking viability post-thawing, particularly when using hemocytometers or automated counting devices.
3. Apoptosis Research:
   • Used to identify apoptotic cells or to study the effects of cell death pathways during drug screening or toxicology studies.
4. Quality Control in Biotech:
   • Used in biotech and pharmaceutical industries to ensure high-quality cell preparations and production cultures.
5. Stem Cell Culture:
   • Useful in stem cell research to determine the viability of stem cells for further use in differentiation or regeneration experiments.

Related Products:

• Gibco™ Trypan Blue Solution, 0.4%
• Thermo Scientific™ Cell Counting Reagents
• Thermo Scientific™ Hemocytometer

The Gibco™ Trypan Blue Solution, 0.4% is an essential and reliable tool for cell viability testing, enabling accurate and fast analysis of viable and non-viable cells. Its ease of use, high quality, and cGMP manufacturing make it an ideal choice for cell culture research, toxicology studies, and quality control in laboratory settings.