Gibco™ HBSS, calcium, magnesium, no phenol red

Gibco™ HBSS (Hanks' Balanced Salt Solution) with Calcium, Magnesium, No Phenol Red is a sterile, ready-to-use buffer solution designed for cell culture applications such as cell washing, transportation, and reagent preparation. This formulation includes calcium and magnesium, making it ideal for maintaining cell integrity during transport and processing.

The absence of phenol red reduces potential interference in sensitive assays, microscopy, and fluorescence-based studies, ensuring optimal performance in primary cell culture, stem cell research, and tissue engineering applications.

Key Features & Benefits

1. Optimized for Cell Culture & Tissue Transport
   • Provides essential ions for maintaining cell viability and osmotic balance.
   • Used as a transport medium to maintain cellular function during processing.
2. Calcium & Magnesium for Enhanced Cell Integrity
   • Supports cell adhesion and intercellular communication.
   • Reduces shear stress during washing steps.
3. Phenol Red-Free for Sensitive Applications
   • Eliminates potential background signal interference in fluorescence-based assays.
   • Suitable for microscopy and immunohistochemistry (IHC) studies.
4. Sterile & Ready-to-Use, cGMP-Compliant
   • Manufactured under ISO 13485 standards ensuring consistent quality and reproducibility.
   • Pre-filtered and sterile, eliminating contamination risks.
5. Long Shelf Life & Room Temperature Stability
   • Shelf life of 36 months from the date of manufacture.
   • Can be stored at 15–30°C, ensuring ease of use in laboratory settings.

Product Specifications

Use Cases & Applications

1. Cell Washing & Transport

• Prepares Cells for Downstream Experiments
  • Maintains osmotic balance and viability during processing.
  • Suitable for washing cells before media changes or cryopreservation.
• Ideal for Tissue Transport & Storage
  • Prevents osmotic shock and cell stress when transporting tissue samples.
  • Used in biopsies and primary cell research workflows.

2. Mammalian Cell Culture & Reagent Preparation

• Serum-Free & Custom Media Preparation
  • Works as a base solution for serum-free and chemically defined media.
  • Suitable for nutrient dilution and preparation of assay reagents.
• Optimized for Cell Attachment & Growth
  • Maintains cell-to-cell interactions in adherent cell cultures.
  • Enhances cell integrity during washing steps and enzymatic treatments.

3. Immunohistochemistry (IHC) & Functional Assays

• Supports Cell Fixation & Staining Protocols
  • Provides a stable, physiologically buffered environment for sample processing.
  • Reduces nonspecific cell lysis during antibody labeling procedures.
• Maintains Cell Functionality in Physiological Conditions
  • Ensures stable pH balance and electrolyte composition.
  • Ideal for preclinical drug screening and cytotoxicity assays.

4. Stem Cell & Organoid Research

• Maintains Cell Viability During Dissociation & Passaging
  • Prevents calcium-dependent junction breakdown during cell handling.
  • Enhances stem cell culture conditions by maintaining ion balance.
• Supports Neural & Cardiac Tissue Engineering
  • Ideal for cardiomyocyte and neural cell research.
  • Works in co-culture systems for tissue regeneration models.

Instructions for Use

General Handling & Preparation

1. Pre-Warm Before Use:
   • Warm HBSS to room temperature or 37°C before application.
2. For Cell Washing & Transport:
   • Gently resuspend cells or tissue in HBSS.
   • Centrifuge at 300-400 x g for 5 minutes, discard the supernatant, and proceed with culture.
3. For Serum-Free Culture & Assay Preparation:
   • Dilute supplements, growth factors, or media components as required.
   • Use as a base solution for custom formulations.

Storage & Handling Instructions

• Storage Conditions:
  • Store at 15–30°C (Room Temperature).
  • Avoid prolonged light exposure to maintain stability.
• Handling Precautions:
  • Use aseptic techniques to prevent contamination.
  • Work in a biosafety cabinet (BSC) when handling sterile media.
• Disposal Guidelines:
  • Dispose of used media according to institutional biosafety protocols.
  • Autoclave if required before disposal.

Why Choose Gibco™ HBSS (With Calcium, Magnesium, No Phenol Red)?

✅ Provides essential ions for maintaining cell viability during transport & washing

✅ Sterile, pre-filtered, ready-to-use formulation ensures reproducible results

✅ Phenol red-free for reduced background in imaging & fluorescence assays

✅ Manufactured under cGMP and ISO 13485 quality standards

✅ Long shelf life (36 months) and stable storage at room temperature

Gibco™ HBSS (With Calcium, Magnesium, No Phenol Red) is an essential solution for primary cell culture, tissue processing, and cell-based assay workflows, ensuring high reproducibility and minimal interference in molecular biology and biopharmaceutical applications.