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Varioskan™ LUX multimode microplate reader (Absorbance, Fluorescence Intensity, Luminescence, Time-resolved Fluorescence)
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Reading Options: Top and Bottom
No. of dispensers: 2
Specifications:
| Application | molecular biology | Warranty | 1 Year |
| Storage Temperature | Ambient | ||
| Product Type | Laboratory Equipment | ||
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Thermo Scientific™ Varioskan™ LUX Multimode Microplate Reader (VL0L0TD0) delivers unmatched multimode detection versatility for a wide range of life science, biochemical, and molecular applications.
Combining Absorbance (UV-Vis), Fluorescence Intensity, Luminescence, and Time-Resolved Fluorescence (TRF) technologies, this compact and modular platform offers high sensitivity, broad dynamic range, and exceptional reproducibility in microplate-based assays.
Optimized for high-throughput workflows, the Varioskan LUX integrates smart automation, dynamic range adjustment, and intuitive SkanIt™ Software, making it the preferred choice for laboratories performing enzyme kinetics, TR-FRET, luminescence-based assays, and quantitative fluorescence studies.
Key Features and Benefits
1. Comprehensive Multimode Detection
- Supports Absorbance (200–1000 nm), Fluorescence Intensity, Luminescence, and Time-Resolved Fluorescence (TRF/TR-FRET).
- Top reading optics ensure optimal detection for homogeneous assays.
- Dual monochromator system provides full wavelength flexibility for excitation and emission measurements.
- Spectral scanning enables real-time wavelength optimization for assay development.
2. High Sensitivity and Wide Dynamic Range
- Fluorescence Sensitivity: <0.4 fmol fluorescein/well (black 384-well plate).
- Time-Resolved Fluorescence Sensitivity: <1 amol/well (white low-volume 384-well plate).
- Luminescence Sensitivity: <7 amol ATP/well (white 384-well plate).
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Dynamic Range:
- Fluorescence Intensity: >6 decades
- Time-Resolved Fluorescence: >6 decades
- Luminescence: >7 decades
- Photometric Accuracy: ±2% (200–399 nm), ±1% (400–1000 nm).
- Precision: SD <0.001 Abs or CV <0.5% at 450 nm.
3. Intelligent Automation and Smart Design
- Automatic dynamic range selection optimizes sensitivity for each well.
- Smart Safety Controls prevent setup and operational errors.
- Self-calibration and diagnostics maintain consistent instrument accuracy.
- Multiple measurement modes: Endpoint, kinetic, spectra, kinetic spectra, and multipoint.
- Orbital shaking and temperature control (ambient +4°C to 45°C) ensure homogeneous and reproducible results.
4. SkanIt™ Software Integration
- Includes SkanIt™ PC Software (Research Edition) for intuitive control and analysis.
- License-free, multi-user installation for maximum accessibility.
- Virtual Pipetting Tool simplifies assay setup and plate mapping.
- Real-time kinetic and spectral curve visualization during measurements.
- Automatic data saving prevents loss from unexpected interruptions.
- Comprehensive data analysis: Blank subtraction, standard curve, dose-response, kinetic modeling, and custom formula calculations.
- Flexible export options: *.xlsx, *.pdf, *.xml, *.txt, with auto-email capability after run completion.
5. Certified and Upgradeable
- HTRF™ certified by Cisbio Bioassays and DLReady™ validated by Promega Corporation.
- Modular, upgradable design allows expansion for AlphaScreen™, AlphaLISA™, or additional multimode functions.
Technical Specifications
| Parameter | Specification |
|---|---|
| Measurement Technologies | Absorbance, Fluorescence Intensity, Luminescence, Time-Resolved Fluorescence |
| Reading Options | Top |
| Wavelength Range | Absorbance: 200–1000 nm; Fluorescence Excitation: 200–822 nm; Emission: 270–840 nm; TRF Excitation: 334 nm; Emission: 400–700 nm; Luminescence: 360–670 nm |
| Measurement Speed | 15 s (96-well), 45 s (384-well), 135 s (1536-well) |
| Dynamic Range | Fluorescence Intensity: >6 decades; TRF: >6 decades; Luminescence: >7 decades |
| Sensitivity | FI: <0.4 fmol fluorescein/well; TRF: <1 amol/well; Luminescence: <7 amol ATP/well |
| Accuracy (Photometric) | ±2% (200–399 nm, 0–2 Abs); ±1% (400–1000 nm, 0–3 Abs) |
| Precision | SD <0.001 Abs or CV <0.5% at 450 nm |
| Linearity | ±2% (96-well: 0–4 Abs; 384-well: 0–3 Abs) |
| Spectral Scanning Speed | <2.2 s/well (400–500 nm, 2 nm step) |
| Light Source | Xenon Flash Lamp |
| Detector Type | Photomultiplier Tube (PMT) |
| Incubation Temperature | Ambient +4°C to 45°C |
| Shaking | Orbital |
| Dimensions (W × D × H) | 53 × 58 × 51 cm (21 × 23 × 20 in) |
| Weight | 57 kg (125 lb) |
| Power Requirements | 100–240 V, 50/60 Hz |
| Plate Compatibility | 6–1536 well plates; μDrop plates |
| Software Edition | SkanIt™ Research Edition |
Applications
- Fluorescence and TRF Assays: FRET/TR-FRET and HTRF-based studies for molecular interaction analysis.
- Luminescent Assays: ATP detection, luciferase, and bioluminescent enzyme analysis.
- Enzyme Kinetics: Real-time enzymatic reaction and inhibition studies.
- Absorbance-Based Quantification: DNA, RNA, and protein measurements.
- Cell-Based Assays: Reporter gene, viability, and cytotoxicity analysis.
- Spectral Scanning: Excitation/emission optimization for customized assays.
- Drug Discovery: High-throughput screening for fluorescence and luminescence assays.
Unique Advantages
- Combines four major detection technologies in one compact instrument.
- Optimized top-reading optics ideal for solution and homogeneous assays.
- Automatic calibration and dynamic range optimization ensure high reproducibility.
- HTRF™ and DLReady™ certifications guarantee assay compatibility.
- Modular and upgradeable for advanced detection modes.
- Paired with SkanIt™ Software for automated data handling and detailed reporting.
- Includes Nunc™ Optical-Bottom Microplates for superior optical performance and cell culture applications.
The Thermo Scientific™ Varioskan™ LUX Multimode Microplate Reader (VL0L0TD0) combines Absorbance, Fluorescence, Luminescence, and Time-Resolved Fluorescence (TRF) technologies into a compact, modular platform designed for precision, flexibility, and reproducibility.
With its smart automation, top-reading optics, and powerful SkanIt™ Software, it delivers high-quality, reproducible results across a variety of research applications, from molecular quantification to FRET/TR-FRET interaction studies.
Complete with Nunc™ 96-Well Optical-Bottom Microplates, the system ensures excellent optical clarity, making it a trusted choice for high-performance biological and biochemical research laboratories.