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Specifications:
| Application | ELISA-Based Assays | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Elisa Kit | Forms | Kit with Various components |
| Product Brand | VIRCELL | ||
| Product Grade | Molecular Biology | ||
The Vircell DENGUE ELISA Kit is a high-performance immunoassay designed for the qualitative detection of antibodies (IgG and IgM) against Dengue virus (DENV) in human serum or plasma. This kit supports accurate serological diagnosis and differentiation between recent and past infections, making it an essential tool for clinical research and epidemiological surveillance of dengue infections.
The assay incorporates both indirect ELISA (IgG) and µ-capture ELISA (IgM) methodologies, ensuring high sensitivity and specificity for detecting immune responses across different stages of infection.
Key Features
- Detection of Dengue virus-specific IgG and IgM antibodies
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Dual methodology:
- Indirect ELISA for IgG
- µ-Capture ELISA for IgM (enhanced specificity for acute infection)
- CE marked for validated performance
- Ready-to-use reagents reduce preparation time
- Break-apart microplate wells for flexible testing volumes
- Compatible with automated ELISA systems
- Includes controls and calibrators for reliable interpretation
- Lyophilized components ensure high stability and consistency
Applications
This kit is ideal for:
- Serological diagnosis of Dengue infection
-
Differentiation of:
- Acute/recent infection (IgM)
- Past infection or immunity (IgG)
- Epidemiological surveillance and outbreak monitoring
- Public health screening programs
-
Differential diagnosis of arboviral diseases such as:
- Chikungunya
- Zika virus
- Other febrile illnesses
Principle of the Assay
Indirect ELISA (IgG)
- Dengue antigens coated on wells bind specific IgG antibodies in the sample
- Enzyme-labelled anti-human IgG binds to the complex
- TMB substrate produces a blue color, turning yellow after stop solution
µ-Capture ELISA (IgM)
- Anti-human IgM antibodies capture IgM from the sample
- Dengue antigen conjugated with peroxidase binds captured IgM
- Color development with TMB substrate indicates presence of IgM
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Name | Vircell DENGUE ELISA Kit |
| Detection Type | Indirect ELISA (IgG), µ-Capture ELISA (IgM) |
| Target | Anti-Dengue Virus IgG / IgM |
| Sample Type | Human serum / plasma |
| Number of Tests | 96 |
| Automation Compatibility | Yes |
| Plate Format | Break-apart wells |
| Detection System | Enzyme (TMB substrate) |
| Certification | CE Marked |
Advantages
- High sensitivity and specificity for dengue serology
- Differentiates acute vs past infection
- Flexible workflow with modular plate design
- Compatible with manual and automated ELISA platforms
- Stable reagents for consistent and reproducible results
- Ideal for high-throughput testing during outbreaks
Related Products
- DENGUE VIRCLIA® (automated immunoassay system)
- ZIKV/DENV/CHIKV Real-Time PCR Kit
- AMPLIRUN® Total ZIKV/DENV/CHIKV Control
- AMPLIRUN® Dengue RNA Control
The Vircell DENGUE ELISA Kit (IgG / IgM Capture) provides a reliable and efficient solution for the serological detection of Dengue virus infection. With its dual detection formats, automation compatibility, and high diagnostic performance, it is well-suited for clinical research laboratories, public health institutions, and infectious disease surveillance programs.
- Detectable Antibody: IgG IgM