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The Roche ABTS™ Solution is a ready-to-use chromogenic substrate for peroxidase-driven indicator reactions in enzyme-linked immunosorbent assay (ELISA). It is formulated with 2,2′-azino-di-[3-ethylbenzthiazoline sulfonate (6)] and hydrogen peroxide in a glycine/citric acid buffer. This solution offers high sensitivity and stability, providing excellent visual results with a distinct bluish-green color.
Key Features:
- Ready-to-Use Format:
- Simplifies workflows by eliminating the need for preparation.
- Pre-formulated in a glycine/citric acid buffer for consistent performance.
- High Sensitivity and Stability:
- Demonstrated superior performance compared to substrates like 5-aminosalicylic acid (5AS), O-phenylenediamine (OPD), and O-tolidine (OT).
- Yields highly sensitive, specific, and reproducible results.
- Distinct Color Development:
- Produces a bluish-green color as the reaction product.
- Ideal for visual and photometric evaluation.
- Compatible with HRP:
- Designed for assays using horseradish peroxidase (HRP) conjugates.
- Optimized for Photometric Evaluation:
- Reaction product absorbs at 405 nm.
- Reference wavelength (≥490 nm) is automatically subtracted by plate readers for background correction.
Specifications:
| Attribute | Details |
|---|---|
| Substrate Type | Chromogenic substrate for HRP |
| Physical Form | Liquid, slightly green |
| Reaction Product Color | Bluish-green |
| Evaluation | Photometric at 405 nm |
| Stability | High stability for reproducible results |
| Packaging | 3 × 100 mL solution |
| Buffer Composition | Glycine/citric acid buffer with H₂O₂ |
| Absorption Reference | 490 nm or higher |
Applications:
- ELISA Assays:
- High-sensitivity detection for HRP-conjugated antibodies or proteins.
- Enzyme Immunoassays:
- Suitable for applications requiring stable and specific colorimetric substrates.
Biochemical/Physiological Actions:
- Substrate Functionality:
- ABTS™ serves as a chromogenic substrate for HRP, enabling the detection of enzyme activity in ELISA assays.
- Delivers a sensitive and specific colorimetric response, enhancing assay reproducibility and clarity.
Advantages:
- Ease of Use: Ready-to-use solution saves time and ensures consistency.
- High Sensitivity: Outperforms other substrates in ELISA applications.
- Distinct Color Development: Bluish-green color enhances visual detection.
- Photometric Compatibility: Optimized for use with plate readers at 405 nm.
Handling and Storage:
- Store as per manufacturer recommendations to maintain reagent stability.
- Note: For life science research only. Not for diagnostic use.
Legal Information:
- ABTS™ is a trademark of Roche.
No resources are currently available for this product.
This will display Shipping & Return.
The Roche ABTS™ Solution is a ready-to-use chromogenic substrate for peroxidase-driven indicator reactions in enzyme-linked immunosorbent assay (ELISA). It is formulated with 2,2′-azino-di-[3-ethylbenzthiazoline sulfonate (6)] and hydrogen peroxide in a glycine/citric acid buffer. This solution offers high sensitivity and stability, providing excellent visual results with a distinct bluish-green color.
Key Features:
- Ready-to-Use Format:
- Simplifies workflows by eliminating the need for preparation.
- Pre-formulated in a glycine/citric acid buffer for consistent performance.
- High Sensitivity and Stability:
- Demonstrated superior performance compared to substrates like 5-aminosalicylic acid (5AS), O-phenylenediamine (OPD), and O-tolidine (OT).
- Yields highly sensitive, specific, and reproducible results.
- Distinct Color Development:
- Produces a bluish-green color as the reaction product.
- Ideal for visual and photometric evaluation.
- Compatible with HRP:
- Designed for assays using horseradish peroxidase (HRP) conjugates.
- Optimized for Photometric Evaluation:
- Reaction product absorbs at 405 nm.
- Reference wavelength (≥490 nm) is automatically subtracted by plate readers for background correction.
Specifications:
| Attribute | Details |
|---|---|
| Substrate Type | Chromogenic substrate for HRP |
| Physical Form | Liquid, slightly green |
| Reaction Product Color | Bluish-green |
| Evaluation | Photometric at 405 nm |
| Stability | High stability for reproducible results |
| Packaging | 3 × 100 mL solution |
| Buffer Composition | Glycine/citric acid buffer with H₂O₂ |
| Absorption Reference | 490 nm or higher |
Applications:
- ELISA Assays:
- High-sensitivity detection for HRP-conjugated antibodies or proteins.
- Enzyme Immunoassays:
- Suitable for applications requiring stable and specific colorimetric substrates.
Biochemical/Physiological Actions:
- Substrate Functionality:
- ABTS™ serves as a chromogenic substrate for HRP, enabling the detection of enzyme activity in ELISA assays.
- Delivers a sensitive and specific colorimetric response, enhancing assay reproducibility and clarity.
Advantages:
- Ease of Use: Ready-to-use solution saves time and ensures consistency.
- High Sensitivity: Outperforms other substrates in ELISA applications.
- Distinct Color Development: Bluish-green color enhances visual detection.
- Photometric Compatibility: Optimized for use with plate readers at 405 nm.
Handling and Storage:
- Store as per manufacturer recommendations to maintain reagent stability.
- Note: For life science research only. Not for diagnostic use.
Legal Information:
- ABTS™ is a trademark of Roche.
No resources are currently available for this product.
This will display Shipping & Return.