Thermo Scientific™ Ponceau S Staining Solution

Ready-to-Use, Reversible Stain for Western Blot Transfer Verification

Thermo Scientific™ Ponceau S Staining Solution is a ready-to-use, reversible protein stain specifically designed to assess transfer efficiency during Western blotting. Compatible with both nitrocellulose and PVDF membranes, this red-pink colorimetric stain provides a rapid, reliable, and nondestructive method to visualize protein bands immediately after transfer.

With a simple protocol and fast reversal using 0.1% NaOH, Ponceau S is ideal for quick blot checks before proceeding with antibody-based detection. It does not alter membrane-bound proteins, ensuring uncompromised downstream immunodetection.

Key Features & Benefits

• ✅ Reversible Staining – Can be removed in less than five minutes with 0.1% NaOH
• ✅ Non-Destructive – Does not interfere with subsequent antibody detection
• ✅ Ready-to-Use Solution – No dilution or preparation required
• ✅ Clear Visual Output – Produces reddish-pink bands easily visible and photographable
• ✅ Compatible with Nitrocellulose & PVDF Membranes – Versatile use in standard Western blot workflows
• ✅ Reliable Transfer Verification – Confirms protein presence and transfer efficiency quickly
• ✅ Minimal Background – Low nonspecific membrane interaction ensures clean visualization

Technical Specifications

Applications

🧪 Western Blotting – Visualize and confirm protein transfer to membranes before immunodetection

🧫 Protein Electrophoresis Workflows – Perform quality control on blots without compromising results

📷 Documentation – Easily photograph reddish-pink bands to record transfer quality

🔬 Routine Laboratory QC – Use as part of blotting SOPs in research and diagnostic labs

Ordering Information

Thermo Scientific™ Ponceau S Staining Solution offers a fast, reliable, and reversible method to confirm protein transfer efficiency during Western blotting. Its ready-to-use formulation, broad membrane compatibility, and non-interference with downstream detection make it an essential QC step for accurate and reproducible protein analysis.