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Thermo Scientific™ Pierce™ Streptavidin Coated Plates
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Specifications:
| Application | Colorimetric Assay, Fluorescence-Based Assay, Chemiluminescent Immunoassay |
| Material | Polystyrene |
| Storage Temperature | 2-8°C |
| Product Type | Plate |
| Product Brand | Thermo Fisher Scientific™ |
| Product Grade | Molecular Biology |
Thermo Scientific™ Pierce™ Streptavidin Coated Plates are ready-to-use, pre-blocked 96-well polystyrene microplates designed for efficient immobilisation of biotinylated antibodies, peptides, oligonucleotides, probes, and other biotin-labelled molecules. These plates provide the high-affinity biotin-binding function required for ELISA, immunoassay development, and other target-specific assay workflows.
Available in clear, white, and black plate formats, they support different detection methods: clear plates for colorimetric assays, white plates for chemiluminescent assays, and black plates for fluorescent assays. The plates are pre-blocked with either Blocker™ BSA Buffer or SuperBlock™ Buffer, helping reduce nonspecific binding and improve assay consistency.
Key Features
- Streptavidin-coated 96-well microplates for biotin-based assay systems
- Ready-to-use and pre-blocked to reduce preparation time
- Approximate binding capacity of ~5 pmol biotin per well
- Suitable for biotinylated antibodies, peptides, probes, and oligonucleotides
- Low nonspecific binding for improved assay performance
- Gentle immobilisation helps preserve protein structure and activity
- Useful for small biotinylated hydrophilic molecules that bind poorly to untreated polystyrene
- Available in clear, white, and black plates for different detection systems
- Choice of Blocker™ BSA Buffer or SuperBlock™ Buffer blocking
- Suitable for ELISA and other target-specific immunoassays
Applications
- ELISA assay development
- Biotin-streptavidin capture assays
- Immunoassays using biotinylated capture antibodies
- Peptide antigen immobilisation
- Oligonucleotide and probe-based assays
- Protein interaction studies
- Antibody screening workflows
- High-affinity immobilisation of biotinylated molecules
- Research immunochemistry and molecular biology workflows
Detection Method Guide
| Plate Colour | Recommended Detection Method | Typical Use |
|---|---|---|
| Clear | Colorimetric | TMB, OPD, ABTS and absorbance-based ELISA assays |
| White | Chemiluminescent | Luminescent ELISA and signal enhancement workflows |
| Black | Fluorescent | Fluorescence-based assays requiring reduced well-to-well crosstalk |
Product Specifications
| Parameter | Specification |
|---|---|
| Brand | Thermo Scientific™ |
| Product Line | Pierce™ |
| Product Name | Streptavidin Coated Plates |
| Product Type | Detection plate, immunoassay plate, ELISA plate |
| Surface Treatment | Streptavidin |
| Binding Property | Standard capacity, affinity |
| Approximate Binding Capacity | ~5 pmol biotin/well |
| Material | Polystyrene |
| Number of Wells | 96 |
| Format Options | Standard or strip plate |
| Colour Options | Clear, white, black |
| Blocking Options | Blocker™ BSA Buffer or SuperBlock™ Buffer |
| Regulatory Status | Research Use Only |
Ordering Information
| Catalog Number | Colour | Format | Plate Blocking | Quantity |
|---|---|---|---|---|
| 15125 | Clear | Standard | Blocker™ BSA Buffer | 5 plates |
| 15219 | Black | Standard | Blocker™ BSA Buffer | 5 plates |
| 15119 | Black | Standard | SuperBlock™ Buffer | 5 plates |
| 15407 | Black | Standard | SuperBlock™ Buffer | 5 plates |
| 15121 | Clear | Strip plate | Blocker™ BSA Buffer | 5 plates |
| 15120 | Clear | Strip plate | SuperBlock™ Buffer | 5 plates |
| 15122 | Clear | Strip plate | SuperBlock™ Buffer | 5 × 5 plates |
| 15124 | Clear | Standard | SuperBlock™ Buffer | 5 plates |
| 15126 | Clear | Standard | SuperBlock™ Buffer | 5 × 5 plates |
| 15405 | Clear | Standard | SuperBlock™ Buffer | 5 plates |
| 15218 | White | Standard | Blocker™ BSA Buffer | 5 plates |
| 15118 | White | Standard | SuperBlock™ Buffer | 5 plates |
Storage & Handling
Store unopened plates at 4°C upon receipt. After opening, unused plates should be placed in a resealable bag with desiccant and stored at 4°C. Plates are shipped at ambient temperature.
Thermo Scientific™ Pierce™ Streptavidin Coated Plates provide a convenient and reliable platform for ELISA and biotin-based assay workflows. Their ready-to-use streptavidin surface, low nonspecific binding, pre-blocked formats, and compatibility with colorimetric, chemiluminescent, and fluorescent detection methods make them a practical choice for laboratories developing sensitive and reproducible immunoassays.
- Plate Type: Standard 96-Well, Clear, with Blocker™ BSA Buffer blocking, 5 Plates Standard 96-Well, Black, with Blocker™ BSA Buffer blocking, 5 Plates Standard 96-Well, Black, with SuperBlock™ Buffer blocking, 5 Plates Standard 384-Well, Black, with SuperBlock™ Buffer blocking, 5 Plates Strip 96-Well, Clear, with Blocker™ BSA Buffer blocking, 5 Plates Strip 96-Well, Clear, with SuperBlock™ Buffer blocking, 5 Plates Strip 96-Well, Clear, with SuperBlock™ Buffer blocking, 5 × 5 Plates Standard 96-Well, Clear, with SuperBlock™ Buffer blocking, 5 Plates Standard 96-Well, Clear, with SuperBlock™ Buffer blocking, 5 × 5 Plates Standard 384-Well, Clear, with SuperBlock™ Buffer blocking, 5 Plates Standard 96-Well, White, with Blocker™ BSA Buffer blocking, 5 Plates Standard 96-Well, White, with SuperBlock™ Buffer blocking, 5 Plates