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Thermo Scientific™ Pierce™ IP Lysis Buffer
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Specifications:
| Application | Protein Assays & Analysis, Colorimetric Assay, Immunoprecipitation (IP) | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Molecular Biology Reagent | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Thermo Scientific™ Pierce™ IP Lysis Buffer is a ready-to-use mammalian whole-cell lysis reagent optimized for preparing cell lysates for immunoprecipitation (IP), co-immunoprecipitation (Co-IP), pull-down assays, protein assays, immunoassays, and protein purification workflows.
Based on a modified RIPA buffer formulation without SDS, Pierce™ IP Lysis Buffer provides effective protein extraction while helping maintain protein complexes for downstream interaction studies. Its moderate-strength formulation solubilizes cytoplasmic, membrane, and nuclear proteins without liberating genomic DNA, reducing sample viscosity and improving handling during IP and pull-down workflows.
Key Features
| Feature | Details |
|---|---|
| Optimized for IP and Co-IP | Designed for immunoprecipitation, co-immunoprecipitation, and pull-down assays |
| Gentle Protein Extraction | Helps maintain protein complexes for interaction studies |
| Broad Protein Recovery | Extracts cytoplasmic, membrane, and nuclear proteins |
| Reduced Sample Viscosity | Does not liberate genomic DNA like harsher lysis buffers |
| SDS-Free Modified RIPA Formula | Provides moderate-strength lysis without disrupting complexes like ordinary RIPA buffer |
| Assay Compatible | Compatible with BCA, 660 nm protein assays, ELISA, Western blot, and reporter assays |
| Ready-to-Use Liquid Format | Convenient prepared formulation for routine laboratory workflows |
| Inhibitor Flexible | Protease and phosphatase inhibitors can be added immediately before use |
Applications
Thermo Scientific™ Pierce™ IP Lysis Buffer is suitable for:
- Immunoprecipitation workflows
- Co-immunoprecipitation assays
- Pull-down assays
- Mammalian whole-cell lysis
- Lysis of plated mammalian cells
- Lysis of cells pelleted from suspension cultures
- Protein-protein interaction studies
- Protein extraction for ELISA and Western blotting
- Protein purification workflows
- Protein concentration assays, including Pierce™ BCA and 660 nm Protein Assays
- Reporter assay-compatible lysate preparation
Product Specifications
| Specification | Details |
|---|---|
| Product Name | Thermo Scientific™ Pierce™ IP Lysis Buffer |
| Product Type | IP Lysis Buffer |
| Product Line | Pierce™ |
| Format | Liquid |
| Buffer Type | Modified RIPA buffer without SDS |
| Recommended Use | Cell lysis for IP, Co-IP, and pull-down workflows |
| Unit Size | Each |
| Storage | 4 °C |
| Research Use | For Research Use Only; not for diagnostic procedures |
Buffer Composition
| Component | Concentration |
|---|---|
| Tris-HCl, pH 7.4 | 25 mM |
| NaCl | 150 mM |
| EDTA | 1 mM |
| NP-40 | 1% |
| Glycerol | 5% |
Important Formulation Note
Pierce™ IP Lysis Buffer does not contain protease or phosphatase inhibitors. For workflows requiring protection from proteolysis or preservation of phosphorylation status, inhibitors such as Thermo Scientific™ Halt™ Protease Inhibitor Cocktail or Halt™ Phosphatase Inhibitor Cocktail may be added immediately before use.
Ordering Information
| Catalog Number | Quantity |
|---|---|
| 87787 | 100 mL |
| 87788 | 250 mL |
Related Products
| Product | Catalog Number |
|---|---|
| M-PER™ Mammalian Protein Extraction Reagent | 78501 |
| RIPA Lysis and Extraction Buffer | 89900 |
Storage & Handling
Upon receipt, store at 4 °C. Keep the bottle tightly closed when not in use and avoid contamination during repeated pipetting or dispensing. Add protease or phosphatase inhibitors fresh before use when required by the experimental workflow.
Research Use Statement
For Research Use Only (RUO). Not for use in diagnostic procedures.
- Pack Size: 100 mL 250 mL