Thermo Scientific™ Maxima H Minus Double-Stranded cDNA Synthesis Kit

The Thermo Scientific™ Maxima H Minus Double-Stranded cDNA Synthesis Kit is a complete and efficient solution for synthesizing high-quality, full-length, double-stranded cDNA from total RNA or mRNA. This all-in-one, single-tube kit eliminates the need for intermediate purification steps such as organic extraction or ethanol precipitation, thereby streamlining the workflow and maximizing cDNA recovery.

The kit incorporates Maxima H Minus Reverse Transcriptase, a robust, RNase H-deficient enzyme with enhanced thermal stability, ensuring accurate and complete first-strand synthesis. Second-strand synthesis follows immediately within the same reaction tube, further simplifying the protocol.

Key Features and Benefits

• Single-tube reaction – no need for purification between first and second strand synthesis
• Efficient production of blunt-ended, full-length cDNA
• RNase H minus RT – preserves RNA template integrity
• Fast protocol – complete procedure in under 2 hours
• Pre-mixed reagents – reduces hands-on time and pipetting errors
• Includes all necessary primers, enzymes, and RNA cleanup reagents

Applications

• Double-stranded cDNA synthesis for downstream cloning
• Construction of cDNA libraries for transcriptomic analysis
• Preparation of templates for in vitro transcription, NGS, or expression studies

Technical Specifications

The Maxima H Minus Double-Stranded cDNA Synthesis Kit offers a highly efficient and streamlined workflow for generating full-length, double-stranded cDNA with minimal sample loss. With pre-mixed components, a reliable reverse transcriptase, and a single-tube format, this kit is an excellent choice for molecular biology laboratories preparing samples for cloning or cDNA library construction.