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Specifications:
| Application | Cell culture | ||
| Storage Temperature | -5 to -30°C | ||
| Product Type | Antibiotic | Forms | lyophilized powder |
| Product Brand | Thermo Fisher Scientific™, Gibco | ||
| Product Grade | Cell Culture | Formula | C₁₇H₂₆N₈O₅ · HCl |
High-Purity Antibiotic for Rapid Selection in Mammalian, Bacterial, and Fungal Systems
Gibco™ Blasticidin S HCl is a potent peptidyl nucleoside antibiotic derived from Streptomyces griseochromogenes. It functions as a broad-spectrum inhibitor of protein synthesis, active in both prokaryotic and eukaryotic cells, including bacteria, mammalian cells, fungi, nematodes, and tumor cell lines. Supplied as a sterile powder (50 mg), it is widely used for antibiotic selection of genetically modified cell lines carrying the BSD or BSR resistance genes.
Blasticidin S acts quickly, inducing cell death within hours, and enables the generation of stable mammalian cell lines in less than one week.
Key Features and Benefits
-
Broad-Spectrum Protein Synthesis Inhibitor
Effective in bacterial, fungal, and mammalian systems, making it ideal for versatile research use. -
Powerful Selection Agent
Rapidly eliminates non-transfected cells. Effective at 1–30 µg/mL in mammalian cells and 25–100 µg/mL in bacterial cultures. -
BSD and BSR Resistance Compatibility
Selects for cells expressing BSD (from Aspergillus terreus) or BSR (from Bacillus cereus), which encode blasticidin deaminase to detoxify blasticidin S. -
High Purity and Long Shelf Life
Supplied as a sterile, high-quality powder with a 48-month shelf life when stored properly. -
Ideal for Custom Use
Powder form allows researchers to prepare concentration-specific working solutions tailored to individual cell lines.
Applications
- Antibiotic selection in mammalian, insect, bacterial, or yeast cells
- Rapid stable cell line generation post-transfection
- Dual-selection systems alongside antibiotics like Geneticin™ or Zeocin™
- Functional gene expression and transgene maintenance
- Protein synthesis inhibition studies
Technical Specifications
| Property | Details |
|---|---|
| Product Name | Blasticidin S HCl, Powder |
| Catalog Number | R21001 |
| Form | Sterile Powder |
| Quantity | 50 mg |
| Molecular Formula | C₁₇H₂₆N₈O₅ · HCl |
| Molecular Weight | 458.90 g/mol |
| CAS Number | 3513-03-9 |
| MDL Number | MFCD02091640 |
| Color | White to off-white |
| Recommended Working Concentration | 1–30 µg/mL (mammalian); 25–100 µg/mL (bacterial) |
| Sterility | Sterile |
| Additives | HEPES |
| Culture Type | Mammalian, Insect, Prokaryotic |
| Storage Conditions | –5°C to –30°C; protect from moisture and light |
| Shipping Conditions | Room temperature |
| Shelf Life | 48 months from date of manufacture |
| Product Type | Antibiotic |
| Product Line | Gibco™ |
| Unit Size | Each |
| Intended Use | For Research Use Only. Not for use in diagnostic procedures. |
Gibco™ Blasticidin S HCl, Powder offers a powerful, rapid-acting antibiotic selection solution for researchers generating stable cell lines or performing antibiotic resistance-based screening. With broad-spectrum activity, compatibility with BSD/BSR resistance genes, and flexible preparation from powder form, it is a critical tool in molecular biology, cell engineering, and microbial research.