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Invitrogen™ Crizanlizumab Humanized Recombinant Human Monoclonal Antibody, 100 µg
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Specifications:
| Application | ELISA-Based Assays | ||
| Storage Temperature | -20°C | ||
| Product Type | Primary Antibodies | Forms | Lyophilized |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ Crizanlizumab Humanized Recombinant Human Monoclonal Antibody is a high-purity biosimilar antibody targeting selectin P (SELP), expressed recombinantly in CHO cells.
This antibody mimics the activity of the therapeutic monoclonal antibody Crizanlizumab, which blocks P-selectin-mediated interactions between endothelial cells, platelets, and leukocytes — key mechanisms underlying inflammation, vascular occlusion, and thrombosis.
With >90% purity confirmed by SDS-PAGE analysis under both reducing and non-reducing conditions, this antibody is ideal for biochemical assays, ELISA, and biosimilar characterization related to P-selectin signaling pathways and adhesion molecule research.
Key Features
- Humanized IgG2κ Isotype: Ensures consistent specificity and reduced immunogenicity.
- Biosimilar Specificity: Recognizes and binds to human P-selectin (SELP).
- Recombinant Production: Expressed in CHO cells under serum-free, GMP-compliant conditions.
- High Purity: >90% purity confirmed by SDS-PAGE analysis.
- Low Endotoxin: <0.01 EU/µg by LAL method, suitable for sensitive functional studies.
- Ready-to-Use Liquid Format: Supplied at 1 mg/mL for direct assay application.
- Broad Application Range: Compatible with ELISA, SDS-PAGE validation, and P-selectin binding assays.
Applications
| Application | Recommended Dilution |
|---|---|
| ELISA | Assay-dependent |
| SDS-PAGE | For purity and structural verification |
Validated Example:
SDS-PAGE analysis of recombinant human SELP protein (expressed in CHO cells) using Crizanlizumab biosimilar antibody (MA5-42133) revealed clear bands under both reducing (R) and non-reducing (NR) conditions, confirming antibody purity and structural integrity.
Product Specifications
| Parameter | Specification |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Human / IgG2, κ |
| Expression System | CHO (Chinese Hamster Ovary) Cells |
| Class | Recombinant Monoclonal Antibody |
| Type | Humanized Antibody |
| Immunogen | Human P-selectin (SELP) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification Method | Protein A Affinity Chromatography |
| Endotoxin Level | < 0.01 EU/µg (LAL method) |
| Storage Buffer | PBS, pH 7.4 |
| Preservative | None |
| Storage Conditions | Short-term: 2–8 °C (1 week); Long-term: –20 to –80 °C (12 months) |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2911276 |
Target Information – SELP (P-Selectin)
Full Name: Selectin P (SELP)
Function:
- Mediates platelet–leukocyte and endothelial–leukocyte adhesion during inflammation and thrombosis.
- Key regulator of vascular occlusion and inflammatory response.
- Implicated in conditions such as sickle cell disease, atherosclerosis, and ischemic injury.
Research Applications:
- P-selectin ligand-binding and inhibition assays.
- Platelet activation and adhesion studies.
- Functional characterization of biosimilar antibodies.
- Development of anti-inflammatory and anti-thrombotic therapeutics.
Quality & Validation
- Purity: Verified by SDS-PAGE (>90%).
- Endotoxin: <0.01 EU/µg by LAL.
- Recombinant Expression: CHO-based production ensures consistent quality and reproducibility.
- Performance: Tested in ELISA and immunoassays targeting SELP.
Storage and Handling
- Storage: Store at –20 °C for up to 12 months. Avoid repeated freeze–thaw cycles.
- Short-Term Use: Keep at 2–8 °C for up to 1 week.
- Handling: Use a manual defrost freezer.
- Format: Ready-to-use liquid, no reconstitution required.
Advantages
- Humanized recombinant IgG2κ antibody for SELP pathway research.
- Low endotoxin level (<0.01 EU/µg) ensures compatibility with in vitro studies.
- Verified purity and structure through SDS-PAGE.
- Ideal for biosimilar, immunological, and vascular biology research.
- Long-term stability and consistency under recommended storage conditions.
The Invitrogen™ Crizanlizumab Humanized Recombinant Monoclonal Antibody is a biosimilar anti-P-selectin (SELP) antibody with humanized IgG2κ structure, expressed in CHO cells and purified to >90% by Protein A chromatography.
With low endotoxin, unconjugated format, and high reproducibility, it serves as a reliable reagent for P-selectin functional studies, biochemical assays, and biosimilar validation workflows.