0
Invitrogen™ Platinum™ PCR SuperMix High Fidelity
Catalog No :
CAS Number :
Brand :
In Stock
Specifications:
| Application | qPCR | ||
| Storage Temperature | -20°C | ||
| Product Type | PCR Master Mix | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ Platinum™ PCR SuperMix High Fidelity is a ready-to-use master mix that streamlines the PCR setup process while providing high-specificity, high-fidelity DNA amplification. This advanced formulation combines Platinum™ Taq DNA Polymerase High Fidelity with a proprietary blend of salts, magnesium, and dNTPs, requiring only the addition of primers and template DNA.
The unique enzyme mix—featuring Taq DNA polymerase and a proofreading Pyrococcus species GB-D polymerase—delivers up to six times greater fidelity than standard Taq polymerase, ensuring accurate amplification of DNA fragments up to 15 kb. Built-in anti-Taq antibodies enable automatic hot-start PCR, enhancing specificity and yield while allowing reaction setup at room temperature.
Key Features
- High Fidelity Amplification: Provides up to 6× greater accuracy than standard Taq DNA polymerase.
- Hot-Start Technology: Anti-Taq antibodies prevent non-specific amplification, improving yield and specificity.
- Long Fragment Capability: Efficiently amplifies DNA fragments up to 15 kb in length.
- Convenient SuperMix Format: Ready-to-use formulation minimizes setup time and reduces pipetting errors.
- Reliable Performance: Consistent amplification across a broad range of templates and GC content.
- Room Temperature Setup: Enables easy and contamination-free reaction assembly without ice.
Specifications
| Parameter | Description |
|---|---|
| Catalog Numbers | 12532016 (100 Reactions), 12532024 (5000 Reactions) |
| Format | SuperMix (Master Mix) |
| Polymerase | Platinum™ Taq DNA Polymerase High Fidelity |
| Fidelity (vs. Taq) | 6× higher |
| Hot Start | Built-in (antibody-mediated) |
| Amplifiable Fragment Size | Up to 15 kb |
| Overhang Type | Mixed blunt and 3′-A overhangs (majority 3′-A) |
| Reaction Speed | Standard |
| Reaction Concentration | 1.1× |
| GC-Rich Template Performance | Low |
| Applications | High-fidelity PCR, Hot-start PCR, Cloning, Mutagenesis |
| Storage Conditions | –20 °C (non-frost-free freezer) |
| Shipping Condition | Wet or Dry Ice |
| Intended Use | For Research Use Only (RUO) |
| Quantity Supplied | 4 × 1.125 mL (per 100 reactions pack) |
Applications
- High-fidelity DNA amplification for cloning and sequencing
- Site-directed mutagenesis requiring proofreading accuracy
- PCR amplification of long DNA fragments (≤15 kb)
- Routine hot-start PCR with reduced nonspecific amplification
- Research protocols demanding reliable and reproducible amplification
The Invitrogen™ Platinum™ PCR SuperMix High Fidelity provides researchers with a powerful, convenient, and accurate PCR solution. By integrating proofreading polymerase technology with built-in hot-start capability, this SuperMix ensures reliable amplification, superior fidelity, and simplified workflow—ideal for cloning, mutagenesis, and other applications requiring precise DNA synthesis.
- Pack Size: 4 x 1.125 mL, Sufficient for 100 Rxns 4 x 56.25 mL, Sufficient for 5000 Rxns