Invitrogen™ Collibri™ Stranded RNA Library Prep Kit for Illumina™ Systems

The Invitrogen™ Collibri™ Stranded RNA Library Prep Kit for Illumina Systems is a high-performance, integrated solution designed for rapid and reliable construction of strand-specific cDNA libraries for next-generation sequencing (NGS). Engineered with Thermo Fisher Scientific’s advanced enzymatic and magnetic bead technologies, this kit ensures superior library quality, reproducibility, and sequencing performance across diverse RNA sample types.

The kit combines the proven performance of SuperScript™ IV Reverse Transcriptase, Dynabeads™ magnetic particles, and Platinum™ SuperFi™ DNA Polymerase, enabling efficient reverse transcription, high-fidelity amplification, and optimized library cleanup. This results in sequencing-ready libraries compatible with all Illumina sequencing platforms, including MiSeq™, NextSeq™, and NovaSeq™ systems.

With a streamlined workflow completed in approximately 4.5 hours, the Collibri Stranded RNA Library Prep Kit supports RNA inputs ranging from 1–25 ng, including challenging samples such as FFPE-derived RNA.

Key Features

Strand-Specific RNA Sequencing

• Preserves transcriptional directionality
• Enables accurate gene expression profiling
• Improves detection of antisense transcripts and overlapping genes

Premium Enzyme Technology for Superior Library Quality

• SuperScript IV Reverse Transcriptase for efficient and robust cDNA synthesis
• Platinum SuperFi DNA Polymerase for ultra-high fidelity amplification
• Dynabeads magnetic particles for efficient purification and cleanup

Fast and Streamlined Workflow

• Complete library preparation in approximately 4.5 hours
• Reduced hands-on time with optimized reaction modules
• Integrated workflow for reverse transcription, fragmentation, ligation, and amplification

Multiplexing Capability for High Throughput Sequencing

• Supports up to 96 multiplexed libraries
• Compatible with:
  • Single-indexed primers
  • Unique dual-indexed primers
• Enables efficient sample batching and cost-effective sequencing

Compatible with Diverse RNA Sample Types

• mRNA-enriched RNA
• Ribodepleted RNA
• Total RNA samples
• FFPE samples
• High-quality and degraded RNA samples

Visual Workflow Tracking for Error Prevention

• Integrated dye-based visual feedback system
• Color changes confirm correct reagent addition
• Improves workflow confidence and reduces preparation errors

Applications

The Collibri Stranded RNA Library Prep Kit is ideal for:

• Gene expression profiling (RNA-Seq)
• Whole transcriptome sequencing
• Differential gene expression analysis
• Alternative splicing analysis
• Transcript isoform discovery
• Gene fusion detection
• Non-coding RNA discovery
• Cancer transcriptomics research
• Biomarker discovery studies

Illumina Sequencer Compatibility

Fully compatible with major Illumina sequencing platforms, including:

• MiSeq™
• NextSeq™ 550
• NextSeq™ 2000
• NovaSeq™ 6000
• HiSeq™ series

Technical Specifications

Workflow Overview

The kit supports a complete RNA-Seq library preparation workflow including:

1. RNA fragmentation (if required)
2. First-strand cDNA synthesis
3. Second-strand synthesis with strand specificity
4. Adapter ligation
5. PCR amplification with indexing primers
6. Magnetic bead cleanup and library purification

Advantages Over Conventional RNA Library Prep Kits

• Faster workflow completion
• Superior enzyme performance and fidelity
• Robust performance with low input RNA
• Compatible with degraded RNA and FFPE samples
• Visual process monitoring for improved reliability
• Seamless compatibility with Illumina sequencing platforms

Recommended Complementary Products

For optimal sequencing workflow, use with:

• Collibri Library Quantification Kit (qPCR-based library quantification)
• Qubit™ dsDNA HS Assay Kit for accurate library quantification
• Qubit™ Flex Fluorometer
• Illumina sequencing reagents and flow cells

The Invitrogen™ Collibri™ Stranded RNA Library Prep Kit for Illumina Systems provides a robust, high-performance solution for strand-specific RNA sequencing library preparation. With its advanced enzyme technology, streamlined workflow, and compatibility with Illumina platforms, the kit enables researchers to generate high-quality, reproducible RNA-Seq libraries for transcriptomics, gene expression studies, and advanced genomics research.