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Invitrogen™ CDP-Star™ Substrate (0.4 mM Ready-to-Use) with Enhancer
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Specifications:
| Application | Western Blot, Southern Blot, Northern Bloth | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Molecular Biology Reagent | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
High-sensitivity, ready-to-use chemiluminescent substrate for alkaline phosphatase detection
The Invitrogen™ CDP-Star™ Substrate (0.4 mM Ready-to-Use) with Sapphire-II™ or Emerald-II™ Enhancer is a chemiluminescent substrate designed for the detection of alkaline phosphatase (AP) in both membrane-based and solution-based assays. Ready-to-use and optimized for protein and nucleic acid blotting on nitrocellulose membranes, this substrate delivers exceptional sensitivity, rapid signal generation, and long-lasting glow emission.
Replacing traditional substrates such as methylumbelliferyl phosphate (MUP) and p-nitrophenyl phosphate (pNPP), CDP-Star™ provides higher sensitivity and superior signal-to-noise performance, ensuring reliable detection even for low-abundance targets.
Key Features & Benefits
- High Sensitivity – Detects AP and AP-labeled molecules with superior signal intensity.
- Fast Signal Development – Maximum light levels achieved in ~10 minutes.
- Long-Lasting Signal – Glow emission persists for several hours for flexible imaging.
- Low Background – Optimized for clear, high-contrast results.
- Versatile – Suitable for Southern, Northern, Western blotting, immunoassays, DNA probe assays, enzyme assays, and reporter gene assays.
- Convenient – Ready-to-use liquid format with included enhancer.
Applications
- Southern Blotting – Sensitive detection of DNA sequences.
- Northern Blotting – RNA detection in gene expression studies.
- Western Blotting – Protein detection with AP-conjugated antibodies.
- Solution-Based Assays – Immunoassays, DNA probe assays, enzyme assays, and reporter gene assays.
Technical Specifications
| Property | Details |
|---|---|
| Detection Method | Chemiluminescence |
| Substrate Type | Alkaline Phosphatase |
| Form | Liquid |
| Format | Bottle |
| Concentration | 0.4 mM |
| Enhancer Options | Sapphire-II™ Enhancer (Cat. No. T2214) / Emerald-II™ Enhancer (Cat. No. T2216) |
| Maximum Light Output | ~10 minutes |
| Glow Duration | Several hours |
| Applications | Southern, Northern, Western blotting; immunoassays; enzyme assays; reporter gene assays |
| Product Line | CDP-Star™, NovaBright™ |
| Shipping Condition | Room Temperature |
| Storage | 4°C |
Ordering Information
| Catalog Number | Description | Quantity |
|---|---|---|
| T2214 | Sapphire-II™ Enhancer | 100 mL |
| T2216 | Emerald-II™ Enhancer | 100 mL |
The Invitrogen™ CDP-Star™ Substrate (0.4 mM Ready-to-Use) with Enhancer delivers unmatched sensitivity, rapid detection, and reliable performance for AP-based detection in blotting and solution assays. Its low background, long glow emission, and ready-to-use format make it an excellent choice for high-quality, reproducible results in molecular biology and immunodetection workflows.
- Enhancer Type: Sapphire-II™ Enhancer Emerald-II™ Enhancer