Gibco™ DMEM/F-12, no glutamine

Gibco™ DMEM/F-12, No Glutamine is a widely used basal medium designed to support the growth and maintenance of a broad range of mammalian cell types. This formulation provides the nutritional balance of a 1:1 mixture of Dulbecco’s Modified Eagle Medium (DMEM) and Ham’s F-12, while allowing researchers full control over glutamine supplementation.

The absence of L-glutamine makes this medium ideal for applications requiring customized glutamine concentrations or the use of stabilized glutamine alternatives such as GlutaMAX™.

Key Features

• 1:1 DMEM and Ham’s F-12 formulation
• High Glucose content
• Contains Sodium Pyruvate
• Includes Phenol Red for pH indication
• Without L-Glutamine
• Without HEPES
• Sterile-filtered
• cGMP-compliant (ISO 13485)
• Research Use or Further Manufacturing (RUO/FMU)

Formulation Details

Included Additives

✔ High Glucose

✔ Phenol Red

✔ Sodium Pyruvate

Excluded Components

✘ No L-Glutamine

✘ No HEPES

✘ No proteins, lipids, or growth factors

Supplementation Requirement:

Typically supplemented with 10% Fetal Bovine Serum (FBS). Glutamine must be added separately if required.

Culture Conditions

• Buffer System: Sodium bicarbonate
• CO₂ Requirement: 5–10% CO₂ incubator
• Storage: 2–8°C (protect from light)
• Shipping: Ambient
• Shelf Life: 12 months from date of manufacture

Suitable Cell Types

This formulation supports the growth of:

• MDCK cells
• Glial cells
• Fibroblasts
• Human endothelial cells
• Rat fibroblasts
• General mammalian cell lines

Technical Specifications

Ordering Information

Gibco™ DMEM/F-12, No Glutamine provides a flexible and dependable basal medium for mammalian cell culture applications where controlled glutamine supplementation is required. By combining the rich nutrient profile of DMEM with the broader component diversity of Ham’s F-12, this formulation ensures robust cell growth while giving researchers greater experimental control.