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Proteintech™ p27/Kip1 Monoclonal Antibody (3F12C10), 150 µL
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Specifications:
| Application | Western Blot | ||
| Storage Temperature | -20°C | ||
| Product Type | Primary Antibodies | Forms | Liquid |
| Product Brand | Proteintech | ||
| Product Grade | Molecular Biology | ||
The Proteintech p27/Kip1 Monoclonal Antibody (Clone 3F12C10) is a highly specific antibody designed for the detection of p27 Kip1 (CDKN1B), a critical regulator of the cell cycle. This antibody recognizes p27 protein derived from a fusion protein immunogen and is validated for Western blot applications, offering excellent sensitivity for studying cell cycle arrest and proliferation control mechanisms.
p27 Kip1 is a key cyclin-dependent kinase inhibitor that regulates the transition from the G1 phase to the S phase of the cell cycle by inhibiting cyclin E-CDK2 and cyclin A-CDK2 complexes. This antibody is particularly valuable in research focused on tumor suppression, cell cycle dysregulation, and cancer biology.
Key Features
- Specific for p27 Kip1 (CDKN1B) cell cycle regulator
- Monoclonal antibody clone 3F12C10
- Reactivity: Human, Mouse, Rat
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Validated for:
- Western Blot (WB): 1:10,000
- High sensitivity detection in protein expression studies
- Supplied as unconjugated liquid antibody
- Purified using Protein G
- Stable formulation in PBS with 50% glycerol
- Preserved with 0.02% sodium azide
Applications
This antibody is ideal for:
- Western blot analysis of p27 expression
- Studies of cell cycle regulation (G1/S checkpoint)
- Research on cyclin-CDK inhibition mechanisms
- Investigation of tumor suppressor pathways
- Analysis of cell proliferation and quiescence
- Cancer research focusing on cell cycle dysregulation
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Name | Proteintech p27/Kip1 Monoclonal Antibody (3F12C10) |
| Catalog Number | 67355-1-Ig |
| Type | Antibody |
| Class | Monoclonal |
| Clone | 3F12C10 |
| Host / Isotype | Mouse / IgG1 |
| Species Reactivity | Human, Mouse, Rat |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1000 µg/mL |
| Amount | 150 µg |
| Volume | 150 µL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Preservative | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
Target Information
p27 Kip1 (CDKN1B) is a cyclin-dependent kinase inhibitor that plays a pivotal role in controlling cell cycle progression. It negatively regulates the activity of cyclin E-CDK2 and cyclin A-CDK2 complexes, thereby preventing premature entry into the S phase.
In addition, p27 interacts with cyclin D-CDK4 complexes, contributing to their assembly and regulation. Its function is tightly controlled by phosphorylation and ubiquitin-mediated degradation, which allows cells to transition from a quiescent state to active proliferation.
Loss or reduced expression of p27 has been associated with tumor progression and poor prognosis in several cancers, making it an important biomarker in oncology and cell biology research.
Availability Note
Important: This product is currently not available in certain regions. Alternative antibodies with similar specifications can be recommended upon request.
Advantages
- High specificity for p27 cell cycle protein
- Strong performance in Western blot applications
- Broad species reactivity for versatile research use
- Reliable monoclonal consistency
- Ideal for cancer and cell cycle studies
The Proteintech p27/Kip1 Monoclonal Antibody (3F12C10) is a robust and reliable tool for researchers studying cell cycle control, tumor suppression, and proliferation pathways. Its validated performance in Western blotting and strong specificity make it a valuable addition to molecular biology and oncology research workflows.