Liofilchem® Campylobacter Blood Free Medium Base, 500g

Liofilchem® Campylobacter Blood Free Medium Base is a selective, blood-free, charcoal-based medium used with Campylobacter CCDA Supplement (ref. 81037) for the isolation and enumeration of thermotolerant Campylobacter species, including C. jejuni, C. coli, and C. lari, from food, environmental, and clinical samples.

This medium, also known as modified Charcoal Cefoperazone Deoxycholate Agar (mCCDA), conforms to ISO 10272 and EN ISO 11133 standards, and is designed to replace blood-containing formulations with charcoal, ferrous sulfate, and sodium pyruvate. It provides improved selectivity and reliability in detecting Campylobacter spp. in compliance with international food safety and public health testing protocols.

Key Features

• ISO 10272-compliant selective medium for Campylobacter detection.
• Blood-free formulation with charcoal and oxygen scavengers.
• Used with Campylobacter CCDA Supplement (ref. 81037) for optimal selectivity.
• Microaerophilic incubation at 41.5°C for 40–48 hours.
• Supports growth of thermotolerant Campylobacter spp. while inhibiting Gram-positive and non-target organisms.
• Suitable for food safety, veterinary, environmental, and clinical microbiology laboratories.

Typical Formula (per litre)

Principle of the Method

• Nutrient-rich components (meat extract, tissue digest, and casein hydrolysate) supply essential growth factors for Campylobacter.
• Charcoal acts as a detoxifying agent by absorbing toxic metabolites.
• Sodium deoxycholate inhibits most Gram-positive bacteria.
• Ferrous sulfate and sodium pyruvate serve as oxygen scavengers, maintaining microaerobic conditions.
• Agar provides the solid medium for colony formation.
• Supplementation with Campylobacter CCDA Supplement (Cefoperazone + Amphotericin B) increases selectivity by suppressing accompanying flora.

Preparation

1. Suspend 45.5 g of powder in 1 L of deionized or distilled water.
2. Heat and stir until fully dissolved.
3. Autoclave at 121°C for 15 minutes.
4. Cool to 45–50°C.
5. Aseptically add 2 vials (10 mL total) of rehydrated Campylobacter CCDA Supplement (ref. 81037).
6. Mix thoroughly and pour into sterile Petri dishes.

Technique

1. Inoculate plates by directly spreading the sample on the agar surface or after enrichment in Bolton Broth (ref. 470340), as per ISO 10272.
2. Incubate at 41.5°C for 40–48 hours under microaerophilic conditions (5–6% O₂, 3–10% CO₂, 84–85% N₂).
3. Examine for typical Campylobacter colonies—greyish, flat, moist with metallic sheen and spreading edges.
4. Confirm suspected colonies on Columbia Blood Agar (ref. 11025) by morphology, motility, oxidase, and temperature tolerance tests.

Quality Control

Appearance (Dehydrated Medium): free-flowing, homogeneous grey-black powder

Appearance (Prepared Medium): opaque, black

Storage and Stability

• Store dehydrated medium at 10–30°C, dry and tightly sealed.
• Store prepared plates at 2–8°C, protected from light.
• Shelf life: 4 years (dehydrated medium).
• Discard if contamination, color change, or deterioration occurs.

Safety and Handling

• For in vitro diagnostic use only.
• Handle using standard microbiological aseptic techniques.
• Consult the Safety Data Sheet (SDS) for proper handling and disposal.
• Dispose of used media and samples in accordance with local biosafety regulations.

Ordering Information

Liofilchem® Campylobacter Blood Free Medium Base (mCCDA) is a robust, ISO-compliant selective medium for the detection and enumeration of thermotolerant Campylobacter spp. from food, environmental, and clinical samples. Its blood-free charcoal formulation, combined with CCDA Supplement, ensures high recovery rates, consistent selectivity, and reproducibility in compliance with ISO 10272 and EN ISO 11133 microbiological testing standards.