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Thermo Scientific™ Pierce™ Peroxidase IHC Detection Kit, 1L
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Specifications:
| Application | Immunohistochemistry | ||
| Storage Temperature | Room Temperature, -20°C, 2-8°C | ||
| Product Type | Assay Kits | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Thermo Scientific™ Pierce™ Peroxidase IHC Detection Kit is a complete, high-sensitivity solution for immunohistochemical (IHC) staining using horseradish peroxidase (HRP)-based detection systems. This kit integrates the Pierce Metal Enhanced DAB Substrate with all essential reagents required for staining, counterstaining, and preservation of tissue samples.
Designed for both paraffin-embedded and frozen tissue sections, the kit delivers exceptional signal intensity with minimal background, enabling clear and reliable visualization of target antigens. Its optimized formulation offers significantly enhanced sensitivity—up to 50× greater than traditional DAB methods—making it ideal for detecting low-abundance targets in research applications.
Key Features
- Ultra-high sensitivity – up to 50× more sensitive than standard DAB
- Produces intense dark brown-black precipitate for superior contrast
- Low background staining with high signal-to-noise ratio
- Ready-to-use two-component substrate system
- Working solution stability of >6 hours at room temperature
- Complete kit for staining, counterstaining, and mounting
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Compatible with:
- Direct HRP-conjugated antibodies
- Avidin-biotin complex (ABC) systems
- Suitable for processing up to ~500 slides
Applications
This kit is ideal for:
- Immunohistochemistry (IHC) staining workflows
- Detection of proteins using HRP-labeled antibodies
- Paraffin-embedded tissue sections
- Frozen tissue sections
- Cytology smears and immunocytochemistry applications
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Research areas including:
- Cancer pathology
- Biomarker detection
- Histopathology studies
- Protein localization in tissues
Kit Components
The kit includes all essential reagents for a complete IHC workflow:
- DAB/Metal Concentrate (10X), 25 mL
- Stable Peroxide Buffer, 250 mL
- Universal Blocking Buffer (TBS), 250 mL
- Peroxidase Suppressor, 2 × 100 mL
- BupH™ Tris Buffered Saline (4 packs)
- Surfact-Amps™ 2 (1% Tween-2), 10 mL
- Harris Modified Hematoxylin, 100 mL (for nuclear counterstaining)
- Mounting Medium, 120 mL
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Name | Pierce™ Peroxidase IHC Detection Kit |
| Catalog Number | 36000 |
| Detection Method | Chromogenic / Colorimetric |
| Substrate | Metal-Enhanced DAB |
| Enzyme System | HRP (Horseradish Peroxidase) |
| Applications | Immunohistochemistry (IHC), Immunodetection |
| Format | Liquid |
| Throughput | Up to ~500 slides |
| Product Line | Pierce™ |
| Storage Conditions | Mixed (−20°C, 4°C, Room Temperature depending on component) |
Advantages
- Complete IHC workflow kit – eliminates need for multiple reagents
- Superior sensitivity and signal clarity
- Reduced background for clean, reproducible results
- Time- and cost-efficient compared to sourcing individual components
- Flexible compatibility with multiple IHC detection systems
The Thermo Scientific™ Pierce™ Peroxidase IHC Detection Kit is a comprehensive and highly sensitive solution for chromogenic immunohistochemistry applications. With enhanced DAB performance, optimized reagents, and complete workflow integration, it is an excellent choice for laboratories seeking reliable, high-contrast tissue staining and efficient IHC processing.