Q-Plex™ Human Cytokine Inflammation Panel 1 (9-Plex)

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Target Analytes (9-Plex Panel)
Interferon Gamma (IFNγ)
Interleukin-1 Alpha (IL-1α)
Interleukin-1 Beta (IL-1β)
Interleukin-2 (IL-2)
Interleukin-4 (IL-4)
Interleukin-6 (IL-6)
Interleukin-8 (IL-8)
Interleukin-10 (IL-10)
Tumor Necrosis Factor Alpha (TNFα)

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Specifications:

Application	Protein Assays & Analysis
Storage Temperature	2-8°C
Product Type	Assay Kits	Forms	Kit with Various components
Product Brand	QUANSYS Biosciences
Product Grade	Molecular Biology

Overview
Resource

The Q-Plex Human Cytokine Inflammation Panel 1 (9-Plex) is a fully quantitative, planar-based multiplex ELISA designed for the simultaneous detection of nine key human inflammatory cytokines in serum or plasma samples. This chemiluminescent assay provides high sensitivity, broad dynamic range, and excellent reproducibility, enabling comprehensive cytokine profiling in a single well using only 25 µL of sample.

The assay allows up to 80 samples to be analyzed per 96-well plate within approximately 2.25 hours, delivering time-efficient and cost-effective multiplex performance compared to traditional single-analyte ELISAs.

Target Analytes (9-Plex Panel)

Interferon Gamma (IFNγ)
Interleukin-1 Alpha (IL-1α)
Interleukin-1 Beta (IL-1β)
Interleukin-2 (IL-2)
Interleukin-4 (IL-4)
Interleukin-6 (IL-6)
Interleukin-8 (IL-8)
Interleukin-10 (IL-10)
Tumor Necrosis Factor Alpha (TNFα)

Assay Principle

The Q-Plex platform uses a planar multiplex format in which nine distinct capture antibodies are precisely adsorbed in a defined spatial array at the bottom of each well. During incubation, target cytokines bind to their respective capture antibodies. A detection antibody mix followed by Streptavidin-HRP enables signal generation.

Upon addition of chemiluminescent substrate, emitted light is captured and quantified using a compatible Q-View imager and analyzed with Q-View Software. The assay delivers highly quantitative results with less than 1% intra-panel cross-reactivity.

Performance Specifications

Analyte	Calibrator Range (pg/mL)	ULOQ (pg/mL)	LLOQ (pg/mL)	LOD (pg/mL)	Inter-Assay CV	Intra-Assay CV	Avg. Linearity
IFNγ	8000 – 10.97	5600	14.3	5.49	8%	4%	97%
IL-1α	4000 – 5.49	2800	7.1	2.74	6%	7%	86%
IL-1β	12226 – 16.77	8558	21.8	8.39	8%	5%	94%
IL-2	5213 – 7.15	3649	9.3	3.58	6%	4%	99%
IL-4	1676 – 2.30	1173	3.0	1.15	6%	6%	94%
IL-6	3000 – 4.12	2100	5.3	2.06	7%	4%	104%
IL-8	2000 – 2.74	1400	3.6	1.37	12%	7%	100%
IL-10	8039 – 11.03	5627	14.3	5.51	7%	5%	103%
TNFα	6500 – 8.92	4522	11.5	4.46	6%	5%	98%

Intra-Assay Cross-Reactivity: All analytes <1%

Validated Sample Types

Human Serum
EDTA Plasma
Heparin Plasma

Kit Contents

Each kit includes:

96-well plate pre-coated with the 9-plex cytokine panel
Calibrator standards
Detection mix
Streptavidin-HRP
Chemiluminescent substrates
Sample diluents
Wash buffer
Instruction manual

Required Instrumentation and Software

All Quansys Q-Plex multiplex assays require the use of Q-View imagers for signal detection and Q-View software for data acquisition and analysis. A fully functional trial version of the software is available, after which a licensed version is required. Performance with third-party imaging systems is not guaranteed.

Applications

Cytokine profiling in inflammation studies
Autoimmune disease research
Infectious disease and sepsis monitoring
Vaccine immunogenicity evaluation
Cancer immunology and immunotherapy research
Translational and biomarker discovery studies

The Q-Plex Human Cytokine Inflammation Panel 1 (9-Plex) provides high analytical sensitivity with low limits of detection across all analytes. The planar multiplex format eliminates bead handling and flow cytometry requirements while maintaining strong precision and minimal cross-reactivity. The small sample requirement makes it suitable for limited-volume clinical samples, and the rapid workflow supports high-throughput laboratory environments.