VWR IPTG (isopropyl-β-D-thiogalactopyranoside) ≥98%
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Specifications:
Application | molecular biology | ||
Storage Temperature | -20°C | ||
Product Type | Laboratory Chemical | Forms | Powder |
Product Brand | VWR | ||
Product Grade | Molecular Biology | Formula | C₉H₁₈O₅S |
VWR IPTG (isopropyl-β-D-thiogalactopyranoside) is a ≥98% purity biochemical reagent widely used in molecular biology and microbiology as an inducer of protein expression. IPTG is a non-metabolizable analog of lactose that specifically induces transcription from lac operon-controlled promoters, making it an essential reagent for recombinant protein production in E. coli expression systems.
Key Features
- High purity IPTG (≥98%) for reproducible results.
- White crystalline powder with stable storage properties.
- Induces lac operon for controlled protein expression in E. coli.
- Non-metabolizable inducer, ensuring sustained expression.
- Supplied in research-grade packaging (10 g, 25 g, 1 kg).
- Low impurity levels: dioxane ≤ 1 ppm, water ≤ 1%.
Specifications
Parameter | Details |
---|---|
Product Name | IPTG (isopropyl-β-D-thiogalactopyranoside) |
CAS Number | 367-93-1 |
Molecular Formula | C₉H₁₈O₅S |
Molecular Weight | 238.31 g/mol |
Assay (Purity) | ≥98% |
Appearance | White crystalline powder |
IR Spectrum | Passes test |
Specific Optical Rotation | –33 to –31 ° (1% in water) |
Dioxane | ≤1 ppm |
Water | ≤1% |
Melting Point | 109.5 – 110.5 °C |
Boiling Point | 402 °C (1013 hPa) |
EINECS Number | 206-703-0 |
MDL Number | MFCD00063273 |
Storage | Freezer (≤ –20 °C recommended) |
Packaging Options | Glass bottles: 10 g (437145X), 25 g (437144N), 1 kg (43714BD) |
Applications
- Protein expression studies in E. coli using lac promoter-controlled systems.
- Molecular cloning for recombinant protein research.
- Induction of fusion proteins (e.g., His-tag, GST-tag, MBP-tagged proteins).
- Synthetic biology and genetic engineering experiments.
- Research in biotechnology, pharmaceuticals, and academic labs.
Research Significance
- IPTG is a gold-standard reagent for inducing recombinant proteins in E. coli.
- Unlike lactose, IPTG is not degraded by cellular metabolism, ensuring constant induction and reproducible protein yields.
- Used extensively in pharmaceutical R&D for recombinant protein production, including enzymes, antibodies, and vaccine candidates.
Ordering Information
Catalog Number | Quantity | Packaging |
---|---|---|
437145X | 10 g | Glass bottle (solids) |
437144N | 25 g | Glass bottle (solids) |
43714BD | 1 kg | Glass bottle |
The VWR IPTG (isopropyl-β-D-thiogalactopyranoside) ≥98% is a high-quality inducer for recombinant protein expression in E. coli, offering reproducibility, purity, and reliability for molecular biology research. With its broad applications in cloning, synthetic biology, and biotechnology, IPTG remains a critical reagent in both academic research and industrial-scale bioproduction.
- Pack Size: 1kg 10g 25g