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Varioskan™ LUX multimode microplate reader (Absorbance, Fluorescence Intensity)
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Reading Options: Top
No. of dispensers: None
Specifications:
| Application | molecular biology | Warranty | 1 Year |
| Storage Temperature | Ambient | ||
| Product Type | Laboratory Equipment | ||
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Thermo Scientific™ Varioskan™ LUX Multimode Microplate Reader is a reliable and flexible instrument designed for bioscience and life science researchers who require high-quality absorbance and fluorescence-based measurements.
Built to deliver accuracy, versatility, and ease of use, this compact multimode reader integrates Absorbance (UV-Vis) and Fluorescence Intensity detection technologies for a wide range of research applications including nucleic acid quantification, protein assays, enzyme kinetics, and cell-based assays.
With intuitive operation, automatic dynamic range selection, and smart safety controls, the Varioskan LUX ensures consistent, reproducible data while reducing setup time and user error.
Key Features and Benefits
1. Flexible Detection Technologies
- Dual detection modes: Absorbance (UV-Vis) and Fluorescence Intensity.
- Monochromator-based wavelength selection offers full flexibility across excitation and emission spectra.
- Spectral scanning mode for assay optimization and peak wavelength identification.
- Top reading option ideal for most biochemical and fluorescent assays.
- Compatible with 6–1536 well plates, including μDrop plates for low-volume samples.
2. Efficient and Reliable Performance
- Automatic dynamic range selection optimizes sensitivity per well.
- Smart Safety Controls prevent setup errors and protect sample integrity.
- Photomultiplier Tube (PMT) detector and xenon flash lamp ensure high sensitivity and precision.
- Precision: SD <0.001 Abs or CV <0.5% at 450 nm (0–3 Abs).
- Fast measurement speed: 15 s for 96-well, 45 s for 384-well, and 135 s for 1536-well plates.
- Wide linear range: ±2% (96-well, 0–4 Abs; 384-well, 0–3 Abs).
3. SkanIt™ Software Integration
- Includes Thermo Scientific™ SkanIt™ Software (Research Edition) for intuitive instrument control and data analysis.
- License-free installation for multiple users and computers.
- Virtual Pipetting Tool simplifies plate layout setup.
- Automatic data backup prevents loss due to power interruptions.
- Built-in calculations for blank subtraction, curve fitting, dose-response, and kinetic analysis.
- Supports multiple export formats: *.xlsx, *.pdf, *.xml, and *.txt.
4. Validated and Certified Performance
- HTRF™ certified by Cisbio Bioassays.
- DLReady™ validated by Promega Corporation.
- Designed for robust performance in routine and advanced research workflows.
Technical Specifications
| Parameter | Specification |
|---|---|
| Measurement Technologies | Absorbance (UV-Vis), Fluorescence Intensity |
| Reading Options | Top |
| Wavelength Range | Absorbance: 200–1000 nm; Fluorescence Excitation: 200–822 nm; Emission: 270–840 nm |
| Measurement Speed | 15 s (96-well), 45 s (384-well), 135 s (1536-well) |
| Dynamic Range | Fluorescence Intensity: >6 decades |
| Sensitivity | <0.4 fmol fluorescein/well (black 384-well plate) |
| Accuracy (Photometric) | ±2% (200–399 nm, 0–2 Abs); ±1% (400–1000 nm, 0–3 Abs) |
| Precision | SD <0.001 Abs or CV <0.5% at 450 nm |
| Linearity | ±2% (96-well 0–4 Abs; 384-well 0–3 Abs at 450 nm) |
| Spectral Scanning Speed | <2.2 s/well (400–500 nm, 1 flash, 2 nm step) |
| Light Source | Xenon Flash Lamp |
| Detector Type | Photomultiplier Tube (PMT) |
| Incubation Temperature | Ambient +4°C to 45°C |
| Shaking | Orbital |
| Interface | PC Software (SkanIt™ Software) |
| Software Languages | English, French, German, Spanish, Portuguese, Italian, Japanese, Chinese |
| Dimensions (W × D × H) | 53 × 58 × 51 cm (21 × 23 × 20 in) |
| Weight | 54 kg (119 lb) |
| Power Requirements | 100–240 V, 50/60 Hz |
| Plate Formats | 6–1536 well plates; μDrop plates |
| Software Type | SkanIt™ PC Software, Research Edition |
Applications
- Nucleic Acid Quantification: DNA, RNA, and protein measurement via absorbance or fluorescence.
- Enzyme Kinetics: Real-time monitoring of enzyme activity and inhibition.
- Fluorescence-Based Assays: FRET, GFP, and other fluorescence intensity applications.
- Protein Analysis: Bradford, BCA, and Lowry assays for protein quantification.
- Cell-Based Studies: Reporter gene expression and viability assays.
- Drug Screening: Quantitative fluorescence assays for compound analysis.
- Molecular Biology: Spectral scanning and kinetic analysis for biochemical characterization.
Unique Advantages
- Combines absorbance and fluorescence technologies in a compact, modular platform.
- Monochromator-based wavelength flexibility for a broad range of assays.
- Automatic calibration, self-diagnostics, and dynamic range selection ensure reliable results.
- Upgradeable design for future addition of luminescence, AlphaScreen™, and TRF technologies.
- Validated HTRF™ and DLReady™ performance for standardized assay compatibility.
- Paired with SkanIt™ Software for seamless workflow management and robust data traceability.
The Thermo Scientific™ Varioskan™ LUX Multimode Microplate Reader (VL0000D0) is a compact, modular, and intelligent platform for absorbance and fluorescence-based measurements in modern laboratories.
With exceptional sensitivity, automatic dynamic range adjustment, and smart workflow automation, it delivers consistent and high-quality data for a wide spectrum of research applications.
Paired with SkanIt™ Software (Research Edition), it ensures easy setup, reliable performance, and accurate data analysis — making it an essential tool for molecular biology, biochemical assays, and life science research.