Sigma-Aldrich Sucrose, ACS reagent

High-Purity Disaccharide for Cell Biology, Cryoprotection, and Biochemical Applications

Sigma-Aldrich™ Sucrose is a high-purity, ACS reagent-grade disaccharide composed of glucose and fructose units. Extensively used in cell culture, plant biology, and enzymology, sucrose serves as a metabolically active energy source, osmolyte, and cryoprotectant. This product is derived from sugar cane and rigorously tested to meet ACS specifications, including low heavy metal content and trace anion/cation screening, ensuring optimal performance across diverse laboratory applications.

Key Features & Benefits

• ACS Reagent Grade – Meets or exceeds American Chemical Society specifications
• High Purity – Low heavy metal and trace ion content for sensitive biochemical workflows
• Multifunctional Cryoprotectant – Enhances cell viability during freezing and thawing processes
• Versatile Metabolite – Active in bacterial, yeast, algal, and mammalian metabolic pathways
• Plant & Microbial Media Supplement – Enhances growth and stress tolerance in culture systems
• Energy & Osmotic Balance Agent – Supports ATP production and maintains cellular homeostasis

Applications

• Cell Biology – Used as a cryoprotectant and osmolyte in mammalian and insect cell cultures
• Plant Research – Supplement for growth media in studies of plant immunity and pathogen response
• Microbial Cultivation – Used in E. coli, yeast (S. cerevisiae), and bacterial culture media
• Enzymatic Assays – Functions as a stabilizer and energy source in biochemical reactions
• Biomedical Research – Supports studies on gut microbiota, neuroimmune interactions, and metabolic regulation
• Photosynthesis & Carbohydrate Metabolism Studies – Central to sucrose transport and storage research in plants

Biochemical/Physiological Actions

• Energy Metabolism – Converted to glucose and fructose for ATP synthesis via glycolysis
• Osmotic Stabilization – Balances intracellular osmolarity under stress or cryogenic conditions
• Cryoprotection – Prevents ice crystal formation and improves post-thaw cell survival
• Biosynthetic Role – Precursor for secondary metabolites in plant and microbial pathways
• Metabolite Classification – Identified as an active metabolite in E. coli, yeast, algae, and mice

Specifications

Sigma-Aldrich™ Sucrose, ACS Reagent Grade is a premium-quality carbohydrate optimized for biochemical, cell culture, and plant biology research. With excellent purity, well-characterized functionality, and broad metabolic relevance, it is an essential component for researchers studying cell energetics, stress response, and metabolite regulation across diverse biological systems.